OCCURRENCE OF DIPHTHAMIDE IN ARCHAEBACTERIA
OCCURRENCE OF DIPHTHAMIDE IN ARCHAEBACTERIA
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DOI：
10.1128/jb.153.3.1342-1347.1983
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发表时间：
1983-01-01
影响因子：
3.2
通讯作者：
BODLEY, JW
中科院分区：
文献类型：
--
作者：
PAPPENHEIMER, AM;DUNLOP, PC;BODLEY, JW
白喉毒素片段A的识别位点的蛋白质合成易位因子中存在的无细胞制剂从古细菌嗜酸热浆菌和嗜盐盐杆菌的性质进行了检查。与早期的工作一致，发现这些生物体的提取物含有一种蛋白因子，它是白喉毒素片段A催化的ADP-核糖基化反应的底物。反应速率为约比真核延伸因子2通常观察到的慢1000倍。白喉（白喉酰胺的脱酰胺形式，即，2-[3-羧酰胺-3-（三甲基氨基）丙基]组氨酸）。盐菌蛋白的量与真核细胞（酿酒酵母和HeLa [人宫颈癌]）的类似制剂的水解产物中发现的量相当。在大肠杆菌蛋白质水解物中未检测到白喉。而古细菌真核细胞延伸因子含有二苯二甲酰胺，它们在其他方面有很大的不同。
The nature of the diphtheria toxin fragment A recognition site in the protein synthesis translocating factor present in cell-free preparations from the archaebacteria Thermoplasma acidophilum and Halobacterium halobium was examined. In agreement with earlier work it was found that extracts from these organisms contain a protein factor which is a substrate for the ADP-ribosylation reaction catalyzed by diphtheria toxin fragment A. The rate of the reaction was .apprx. 1000 times slower than that typically observed with eukaryotic elongation factor 2. The presence of diphthine (the deamidated form of diphthamide, i.e., 2-[3-carboxyamide-3-(trimethylammonio)propyl]histidine) was demonstrated in acid hydrolysates of H. halobium protein in amounts comparable to those found in hydrolysates of similar preparations from eukaryotic cells (Saccharomyces cerevisiae and HeLa [human cervical carcinoma]). Diphthine could not be detected in hydrolysates of protein from the eubacterium Escherichia coli. Whereas archaebacterial eukaryotic elongation factors contain diphthamide, they differ importantly in other respects.