Concordance between p53 protein overexpression and gene mutation in a large series of common human carcinomas
Concordance between p53 protein overexpression and gene mutation in a large series of common human carcinomas
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DOI：
10.1016/s0046-8177(96)90282-8
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发表时间：
1996-10-01
期刊：
影响因子：
3.3
通讯作者：
Iacopetta, BJ
中科院分区：
文献类型：
--
作者：
Soong, R;Robbins, PD;Iacopetta, BJ
将 p53 蛋白的免疫组织化学 (IHC) 检测与许多结直肠癌 (n = 100)、乳腺癌 (n = 92)、子宫内膜癌 (n = 122) 和胃癌 (n = 116) 中 P53 基因突变的存在进行比较。两种市售抗体 DO7 和 CM1 用于石蜡包埋组织切片的 MC 分析。使用聚合酶链反应-单链构象多态性（PCR-SSCP）对冷冻和石蜡包埋的肿瘤样本中的基因突变进行筛查。每种肿瘤类型的 DO7 或 CM1 核染色频率分别为结直肠癌（36%、23%）；乳房（15%、19%）；子宫内膜 (21%, 33%) 和胃 (23%,-)。对于分析的 314 个肿瘤，两种抗体的核染色总体相关性为 90%。在 17% 的乳腺癌和 5% 的胃癌中观察到 DO7 的细胞质染色，在 17% 的乳腺癌和 54% 的子宫内膜癌中观察到 CM1 的细胞质染色。 p53 基因突变存在于 39% 的结直肠癌、28% 的乳腺癌、13% 的子宫内膜癌和 25% 的胃癌中。 p53核过表达与基因突变（均为阳性或均为阴性）之间的一致性在结直肠癌中为68%，在乳腺癌中为79%，在子宫内膜癌中为76%，在胃癌中为73%。这项研究提供了进一步的证据，表明 MC 检测 p53 蛋白积累并不总是表明基因突变的存在，反之亦然。在大约 20% 至 30% 的研究肿瘤中观察到不一致的结果，这突出表明在评估 p53 状态与肿瘤行为之间的关系时需要仔细表征 p53 基因和蛋白质的改变。版权所有 (C) 1996 W.B.桑德斯公司
Immunohistochemical (IHC) detection of p53 protein was com pared with the presence of P53 gene mutation in many colorectal (n = 100), breast (n = 92), endometrial (n = 122), and gastric (n = 116) carcinomas. Two commercially available antibodies, DO7 and CM1, were used for MC analysis of paraffin-embedded tissue sections. Screening for gene mutations in frozen and paraffin-embedded tumor samples was carried out using polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP). The frequency of nuclear staining with DO7 or CM1 for each tumor type, respectively was colorectal (36%, 23%); breast (15%, 19%); endometrial (21%, 33%), and gastric (23%,-). Overall correlation between the two antibodies for nuclear staining was 90% for the 314 tumors analyzed. Cytoplasmic staining was observed with DO7 in 17% of breast and 5% of gastric carcinomas and with CM1 in 17% of breast and 54% of endometrial carcinomas. p53 gene mutation was found in 39% of colorectal, 28% of breast, 13% of endometrial, and 25% of gastric cancers. The concordance between p53 nuclear overexpression and gene mutation (both positive or both negative) was 68% for colorectal, 79% for breast, 76% for endometrial, and 73% for gastric carcinomas. This study provides further evidence that MC detection of p53 protein accumulation does not always indicate the presence of a gene mutation and vice versa. Discordant results were observed in approximately 20% to 30% of the tumors studied, highlighting the need for careful characterization of both p53 gene and protein alterations when assessing the relationship between p53 status and tumor behavior. Copyright (C) 1996 by W.B. Saunders Company