The design and application of genetically encodable biosensors based on fluorescent proteins.
The design and application of genetically encodable biosensors based on fluorescent proteins.
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DOI：
10.1007/978-1-62703-622-1_1
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Zhang, Jin
中科院分区：
文献类型：
--
作者：
Newman, Robert H;Zhang, Jin
为了跟踪内源性细胞环境中细胞信号分子的活性，研究人员开发了一套不同的遗传编码荧光生物传感器。这些传感器可以靶向特定的亚细胞区域，以真实的实时监测给定信号分子的特定库，依赖于传感器结构域中的构象变化来改变绿色荧光蛋白（GFP）家族成员的生物物理性质。在这一介绍性章节中，我们首先讨论了绿色荧光蛋白家族成员的属性，然后将我们的注意力转向设计和应用遗传编码的荧光生物传感器活细胞成像。
To track the activity of cellular signaling molecules within the endogenous cellular environment, researchers have developed a diverse set of genetically encodable fluorescent biosensors. These sensors, which can be targeted to specific subcellular regions to monitor specific pools of a given signaling molecule in real time, rely upon conformational changes in a sensor domain to alter the photophysical properties of green fluorescent protein (GFP) family members. In this introductory chapter, we first discuss the properties of GFP family members before turning our attention to the design and application of genetically encodable fluorescent biosensors to live cell imaging.