Control of gene expression by CRISPR-Cas systems.
Control of gene expression by CRISPR-Cas systems.
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DOI：
10.12703/p5-47
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Marraffini LA
中科院分区：
文献类型：
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作者：
Bikard D;Marraffini LA
簇状规则间隔短回文重复序列(CRISPR)基因座及其相关的CA(CRISPR相关)基因在细菌和古菌中提供了对病毒(噬菌体)和其他可移动遗传元件的适应性免疫。虽然早期的大部分工作主要是CRISPR-Cas系统指导噬菌体或质粒DNA切割的例子，但最近的研究揭示了CRISPR-Cas基因座可能参与基因调控的更复杂的情况。本文综述了这些基因座在基因表达调控中的作用，以及利用工程CRISPR-CAS系统进行基因调控的最新进展。
Clustered regularly interspaced short palindromic repeats (CRISPR) loci and their associated cas (CRISPR-associated) genes provide adaptive immunity against viruses (phages) and other mobile genetic elements in bacteria and archaea. While most of the early work has largely been dominated by examples of CRISPR-Cas systems directing the cleavage of phage or plasmid DNA, recent studies have revealed a more complex landscape where CRISPR-Cas loci might be involved in gene regulation. In this review, we summarize the role of these loci in the regulation of gene expression as well as the recent development of synthetic gene regulation using engineered CRISPR-Cas systems.