The Protective Role of the Pericellular Matrix in Chondrocyte Apoptosis
The Protective Role of the Pericellular Matrix in Chondrocyte Apoptosis
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DOI：
10.1089/ten.tea.2010.0601
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发表时间：
2011-08-01
影响因子：
4.1
通讯作者：
Zhang, Zijun
中科院分区：
文献类型：
--
作者：
Peters, H. Charlie;Otto, Thomas J.;Zhang, Zijun
简介：本研究的目的是量化的软骨细胞凋亡中的细胞周基质（PCM）的作用，使用软骨球，这是一个软骨功能单位，包括软骨细胞及其相关的PCM.Methods：软骨细胞和软骨球酶促分离从人关节软骨和暴露于碘乙酸（MIA）和星形孢菌素诱导凋亡。软骨素的定义是存在VI型胶原蛋白，PCM的基本成分。软骨细胞和软骨球的凋亡通过流式细胞术与膜联蛋白V结合来测量，并通过末端dUTP缺口末端标记染色来验证。在一个单独的实验中，分离的软骨细胞进行处理，可溶性VI型胶原蛋白，前或后与MIA凋亡诱导，并通过LDH和末端dUTP缺口末端标记staining.Results的活性测定细胞死亡：与MIA处理的软骨细胞发生27%的细胞死亡，与12%的软骨。在用MIA处理时，9%的软骨细胞发生凋亡，相比之下，只有1.6%的软骨球发生凋亡。类似地，星形孢菌素诱导软骨细胞中13%的凋亡，而软骨球中为3%。VI型胶原蛋白的预孵育有效地防止软骨细胞从MIA诱导的细胞死亡。MIA诱导软骨细胞凋亡后，VI型胶原处理未能使软骨细胞免于死亡。结论：软骨细胞固有的微环境PCM对软骨细胞凋亡具有保护作用。VI型胶原蛋白是PCM的功能成分，有助于软骨细胞的存活。
Introduction: This study was designed to quantify the role of the pericellular matrix (PCM) in chondrocyte apoptosis using chondrons, which are a cartilage functional unit including a chondrocyte and its associated PCM.Methods: Chondrocytes and chondrons were enzymatically isolated from human articular cartilage and exposed to monosodium iodoacetate (MIA) and staurosporine for apoptosis induction. Chondrons were defined by the presence of type VI collagen, a basic component of the PCM. Apoptosis of chondrocytes and chondrons was measured with annexin V binding by flow cytometry and verified with terminal dUTP nick end-labeling staining. In a separate experiment, isolated chondrocytes were treated with soluble type VI collagen, before or after apoptosis induction with MIA, and cell death was measured by the activity of LDH and terminal dUTP nick end-labeling staining.Results: Chondrocytes treated with MIA incurred 27% cell death, compared with 12% in chondrons. On treating with MIA, 9% of chondrocytes underwent apoptosis, compared with only 1.6% of chondrons. Similarly, staurosporine induced 13% apoptosis in chondrocytes, whereas it was 3% in chondrons. Preincubation of type VI collagen effectively prevented chondrocytes from MIA-induced cell death. After apoptosis was induced with MIA, however, treatment with type VI collagen failed to rescue chondrocytes from death.Conclusion: The PCM, a native microenvironment of chondrocytes, protects chondrocytes from apoptosis. Type VI collagen is a functional component of the PCM that contributes to the survival of chondrocytes.