Fungal elicitors stimulate biomass and active ingredients accumulation in Dendrobium catenatum plantlets
Fungal elicitors stimulate biomass and active ingredients accumulation in Dendrobium catenatum plantlets
复制标题
真菌诱导子刺激链状石斛兰幼苗中生物量和活性成分的积累
DOI：
10.2478/s11756-018-0091-9
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发表时间：
2018-10-01
期刊：
影响因子：
1.5
通讯作者：
Qin, Luping
中科院分区：
文献类型：
--
作者：
Zhu, Bo;Wu, Lingshang;Qin, Luping
内生真菌已被广泛用作生物诱导子来刺激植物细胞、组织和器官培养物中的生长和代谢物的产生。在此，从四种内生真菌 DO14 (Pestalotiopsis sp.)、DO18 (Talaromyces sp.)、DO19 (Xylariaceae sp.) 和 DO120 (Hypoxylon sp.) 制备菌丝体提取物 (ME)、上清液 (SL)、乙醇沉淀物 (ES) 和蛋白质多糖组分 (PPF)，并将其应用于宿主链状石斛。经过8周的共培养，ME、ES和PPF对生物量产量和活性成分含量表现出强烈的刺激作用。在这三个诱导子中，PPF 被发现是导致链状 D. catenatum 中生物量和活性成分增强的活性成分。在 DO14 的 240 mg/L PPF 处理下，我们实现了最大茎鲜重 (FW) 和叶 FW。然而，为了最大限度地提高多糖、柚皮素和 schaftoside 的产量，只需从 DO14 中提取 60 毫克/升的 PPF。 DO18、DO19 和 DO120 的 PPF 显示出不同的效果。在30 mg/L处理下，乙醇提取物、总黄酮和总酚含量增加最多。这些结果表明，真菌激发子PPF可用于工业化生产高质量链状D. catenatum幼苗，并可作为其他植物生长的广泛微生物肥料资源。
Endophytic fungi have been widely used as biotic elicitors to stimulate the growth and production of metabolites in plant cells, tissues and organ cultures. Here, mycelium extract (ME), supernatant liquor (SL), ethanol sediment (ES) and protein-polysaccharide fraction (PPF) were prepared from four endophytic fungi, DO14 (Pestalotiopsis sp.), DO18 (Talaromyces sp.), DO19 (Xylariaceae sp.) and DO120 (Hypoxylon sp.), and applied to their host Dendrobium catenatum. After 8 weeks of co-culturing, ME, ES and PPF exhibited strong stimulation on biomass yields and contents of active ingredients. Among the three elicitors, PPF was found to be the active constituent responsible for the enhanced biomass and active ingredients in D. catenatum. Under the treatment of 240 mg/L PPF from DO14, we achieved maximum stem fresh weight (FW) and leaf FW. However, to maximize the productions of polysaccharides, naringenin and schaftoside one need only 60 mg/L of PPF from DO14. PPF from DO18, DO19 and DO120 showed different effects. Under 30 mg/L treatment, the ethanol extractives, total flavonoids and total phenols contents increased most. These results indicate that fungal elicitor PPFs can be used for industrial production of high quality D. catenatum seedlings and may be served as a broad microbial fertilizer resource for other plant growth.