Function of a novel GDSL-type pepper lipase gene, CaGLIP1, in disease susceptibility and abiotic stress tolerance
Function of a novel GDSL-type pepper lipase gene, CaGLIP1, in disease susceptibility and abiotic stress tolerance
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DOI：
10.1007/s00425-007-0637-5
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发表时间：
2008-02-01
期刊：
影响因子：
4.3
通讯作者：
Hwang, Byung Kook
中科院分区：
文献类型：
--
作者：
Hong, Jeum Kyu;Choi, Hyong Woo;Hwang, Byung Kook
GDSL型脂肪酶是一种水解酶，其氨基酸序列含有具有活性丝氨酸（Ser）的五肽基序（Gly-X-Ser-X-Gly）。辣椒 GDSL 型脂肪酶 (CaGLIP1) 基因是从受野油菜黄单胞菌感染的辣椒叶组织中分离出来并进行功能表征的。囊泡 (Xcv)。 CaGLIP1蛋白位于拟南芥根的维管组织中。在与 Xcv 的相容相互作用过程中，CaGLIP1 基因优先在辣椒叶片中表达。水杨酸、乙烯和茉莉酸甲酯处理可诱导辣椒叶片中 CaGLIP1 基因的表达。硝普钠、甲基紫精、高盐、甘露醇介导的脱水和受伤也会诱导辣椒叶组织中早期和瞬时的 CaGLIP1 表达。辣椒中病毒诱导的 CaGLIP1 基因沉默增强了对 Xcv 的抗性，同时抑制了碱性 PR1 (CaBPR1) 和防御素 (CaDEF1) 基因的表达。大肠杆菌中产生的 CaGLIP1 脂肪酶可水解短链和长链硝基苯酯的底物。 CaGLIP1过表达的拟南芥对短链和长链硝基苯酯的水解活性增强，并且对细菌病原体丁香假单胞菌的敏感性增强。番茄和生物营养卵菌寄生玻璃囊孢菌 (Hyaloperonospora parasitica)。 SA 诱导的 AtPR1 和 AtGST1 表达在 CaGLIP1 过表达植物中也因 SA 的应用而延迟。在种子发芽和植物生长过程中，CaGLIP1转基因植物表现出耐旱性以及干旱和脱落酸（ABA）诱导基因AtRD29A、AtADH和AtRab18的差异表达。 ABA 处理差异调节野生型和 CaGLIP1 转基因拟南芥中的种子萌发和基因表达。 CaGLIP1 的过度表达还调节葡萄糖和氧化应激信号传导。总之，这些结果表明 CaGLIP1 调节疾病易感性和非生物胁迫耐受性。
GDSL-type lipase is a hydrolytic enzyme whose amino acid sequence contains a pentapeptide motif (Gly-X-Ser-X-Gly) with active serine (Ser). Pepper GDSL-type lipase (CaGLIP1) gene was isolated and functionally characterized from pepper leaf tissues infected by Xanthomonas campestris pv. vesicatoria (Xcv). The CaGLIP1 protein was located in the vascular tissues of Arabidopsis root. The CaGLIP1 gene was preferentially expressed in pepper leaves during the compatible interaction with Xcv. Treatment with salicylic acid, ethylene and methyl jasmonate induced CaGLIP1 gene expression in pepper leaves. Sodium nitroprusside, methyl viologen, high salt, mannitol-mediated dehydration and wounding also induced early and transient CaGLIP1 expression in pepper leaf tissues. Virus-induced gene silencing of CaGLIP1 in pepper conferred enhanced resistance to Xcv, accompanied by the suppressed expression of basic PR1 (CaBPR1) and defensin (CaDEF1) genes. The CaGLIP1 lipase produced in Escherichia coli hydrolyzed the substrates of short and long chain nitrophenyl esters. The CaGLIP1-overexpressing Arabidopsis exhibited enhanced hydrolytic activity toward short and long chain nitrophenyl ester, as well as enhanced susceptibility to the bacterial pathogen Pseudomonas syringae pv. tomato and the biotrophic oomycete Hyaloperonospora parasitica. SA-induced expression of AtPR1 and AtGST1, also was delayed in CaGLIP1-overexpressing plants by SA application. During seed germination and plant growth, the CaGLIP1 transgenic plants showed drought tolerance and differential expression of drought- and abscisic acid (ABA)-inducible genes AtRD29A, AtADH and AtRab18. ABA treatment differentially regulated seed germination and gene expression in wild-type and CaGLIP1 transgenic Arabidopsis. Overexpression of CaGLIP1 also regulated glucose- and oxidative stress signaling. Together, these results indicate that CaGLIP1 modulates disease susceptibility and abiotic stress tolerance.