Experimental Lachesis muta rhombeata envenomation and effects of soursop (Annona muricata) as natural antivenom
Experimental Lachesis muta rhombeata envenomation and effects of soursop (Annona muricata) as natural antivenom
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DOI：
10.1186/s40409-016-0067-6
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发表时间：
2016-01-01
影响因子：
2.4
通讯作者：
Arantes, Eliane Candiani
中科院分区：
文献类型：
--
作者：
Cremonez, Caroline Marroni;Leite, Flávia Pine;Arantes, Eliane Candiani
背景资料：在巴西北部和东北部的大西洋森林中，当地居民经常使用番荔枝（Annona muricata L.）的果汁和叶的水提取物。治疗红纹穆塔毒刺。在这些地区，毒液中毒是一个相关的健康问题，特别是由于其严重性，因为抗蛇毒血清的生产和分销在这些地区是有限的。本研究的目的是评估使用番荔枝叶提取物和其果汁的相关性对envenomation由Lachesis穆塔rhombeata.Methods：我们评估了生化，血液学和止血参数，血压，炎症过程和致死率由Lachesis穆塔rhombeata蛇毒诱导。我们还评估了叶水提物（AmL）和果汁（AmJ）的作用。muricata对注射L. M.结果：菱贝蛇毒（LmrV）可引起总蛋白、白蛋白和葡萄糖含量下降，肌酸激酶、天冬氨酸转氨酶和尿素含量升高。它引起血液浓缩，随后红细胞压积降低，凝血酶原时间和部分凝血活酶时间增加，血压降低。LmrV诱导白细胞介素-6的释放，中性粒细胞的增加和血清蛋白谱的变化，急性炎症过程的特征。注射LmrV和用AmJ和AmL处理或未处理的组的LD 50值相似。两种治疗均对维持血糖、尿素和凝血参数发挥作用，并对肌毒性发挥保护作用。结论：AmJ和AmL治疗具有一定的降压作用，但可能会加强蛇毒的某些作用。因此，对A. muricata是必要的，使其能够用作天然抗蛇毒血清，为毒蛇咬伤。
Background: In the Atlantic forest of the North and Northeast regions of Brazil, local population often uses the fruit juice and the aqueous extract of leaves of soursop (Annona muricata L.) to treat Lachesis muta rhombeata envenomation. Envenomation is a relevant health issue in these areas, especially due to its severity and because the production and distribution of antivenom is limited in these regions. The aim of the present study was to evaluate the relevance of the use of soursop leaf extract and its juice against envenomation by Lachesis muta rhombeata.Methods: We evaluated the biochemical, hematological and hemostatic parameters, the blood pressure, the inflammation process and the lethality induced by Lachesis muta rhombeata snake venom. We also assessed the action of the aqueous extract of leaves (AmL) and juice (AmJ) from A. muricata on the animal organism injected with L. m. rhombeata venom (LmrV) in the laboratory environment.Results: LmrV induced a decrease of total protein, albumin and glucose; and increase of creatine kinase, aspartate aminotransferase, and urea concentrations. It provoked hemoconcentration followed by reduction of hematocrit, an increase in prothrombin time and partial thromboplastin time and a decrease of the blood pressure. LmrV induced the release of interleukin-6, an increase in neutrophils and changes in the serum protein profile, characteristic of the acute inflammatory process. LD50 values were similar for the groups injected with LmrV and treated or untreated with AmJ and AmL. Both treatments play a role on the maintenance of blood glucose, urea and coagulation parameters and exert a protective action against the myotoxicity. However, they seem to worsen the hypotension caused by LmrV.Conclusion: The treatments with AmJ and AmL present some beneficial actions, but they might intensify some effects of the venom. Therefore, additional studies on A. muricata are necessary to enable its use as natural antivenom for bushmaster snakebite.