DAMB, a novel dopamine receptor expressed specifically in Drosophila mushroom bodies
DAMB, a novel dopamine receptor expressed specifically in Drosophila mushroom bodies
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DOI：
10.1016/s0896-6273(00)80139-7
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发表时间：
1996-06-01
期刊：
影响因子：
16.2
通讯作者：
Davis, RL
中科院分区：
文献类型：
--
作者：
Han, KA;Millar, NS;Davis, RL
在果蝇蘑菇体中，触发嗅觉学习的生化级联反应的调节性神经递质仍然未知。为了确定可能发挥这一作用的分子，利用聚合酶链式反应(PCR)和单链构象多态性分析克隆了可能的生物胺受体。通过序列分析和药理学鉴定，一个新的受体DAMB被鉴定为多巴胺D1R。原位杂交和免疫组织化学分析表明，DAMB在蘑菇体中的表达高度丰富，其模式与Rutabaga编码的腺酰环化酶一致。DAMB和环化酶的空间共表达，以及DAMB介导多巴胺诱导的cAMP增加的能力，使该受体成为启动学习的生化级联反应的一个有吸引力的候选受体。
The modulatory neurotransmitters that trigger biochemical cascades underlying olfactory learning in Drosophila mushroom bodies have remained unknown. To identify molecules that may perform this role, putative biogenic amine receptors were cloned using the polymerase chain reaction (PCR) and single-strand conformation polymorphism analysis. One new receptor, DAMB, was identified as a dopamine D1 receptor by sequence analysis and pharmacological characterization. In situ hybridization and immunohistochemical analyses revealed highly enriched expression of DAMB in mushroom bodies, in a pattern coincident with the rutabaga-encoded adenylyl cyclase. The spatial coexpression of DAMB and the cyclase, along with DAMB's capacity to mediate dopamine-induced increases in cAMP make this receptor an attractive candidate for initiating biochemical cascades underlying learning.