piRNA, the new non-coding RNA, is aberrantly expressed in human cancer cells
piRNA, the new non-coding RNA, is aberrantly expressed in human cancer cells
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PiRNA，一种新的非编码RNA，在人类癌细胞中异常表达
DOI：
10.1016/j.cca.2011.05.015
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发表时间：
2011-08-17
影响因子：
5
通讯作者：
Li, Qing-Ning
中科院分区：
文献类型：
--
作者：
Cheng, Jia;Guo, Jun-Ming;Li, Qing-Ning
背景：Piwi相互作用RNAs(PiRNAs)是一类新的非编码单链RNA。它们参与生殖系发育，沉默自私的DNA元件，并维持生殖系DNA的完整性。方法：通过基因芯片筛选和实时定量逆转录聚合酶链式反应技术鉴定piRNAs与肿瘤发生的关系。将Pir-651抑制剂导入胃癌细胞，观察其对细胞生长的影响。细胞周期分析揭示Pir-651在胃癌发生中的细胞机制。结果：Pir-651在胃癌组织中表达上调，与配对的非癌组织相比差异有统计学意义。Pir-651表达水平与肿瘤分期有关(P=0.032)。Pir-651在胃癌、结肠癌、肺癌和乳腺癌组织中的表达高于配对的非癌组织。Pir-651在胃、肺、间皮、乳腺、肝脏和宫颈癌等多种癌细胞系中均有上调表达。结论：Pir-651可能参与了胃癌等多种肿瘤的发生发展，是一种潜在的肿瘤标志物。(C)2011爱思唯尔B.V.保留所有权利。
Background: Piwi-interacting RNAs (piRNAs) are a novel class of non-coding single strand RNAs. They are involved in germline development, in silencing of selfish DNA elements, and in maintaining germline DNA integrity. The relationship between piRNAs and carcinogenesis has not been shown yet.Methods: The relationship between piRNAs and carcinogenesis was identified by microarray screening and real-time quantitative reverse transcription-polymerase chain reaction technology. The piR-651 inhibitor was transfected into gastric cancer cells to assess its influence on cell growth. Cell cycle analysis was used to reveal the cellular mechanisms of piR-651 in the genesis of gastric cancer.Results: piR-651 expression was upregulated in gastric cancer tissues compared with paired non-cancerous tissues. The levels of piR-651 were associated with TNM stage (P = 0.032). The expression of piR-651 in gastric, colon, lung, and breast cancer tissues was higher than that in paired non-cancerous tissues. The upregulated expression of piR-651 was confirmed in several cancer cell lines including gastric, lung, mesothelium, breast, liver, and cervical cancer cell lines. The growth of gastric cancer cells was inhibited by a piR-651 inhibitor and arrested at the G(2)/M phase.Conclusion: piR-651 might be involved in the development of gastric cancer and other cancers, and is a potential marker for cancer diagnosis. (C) 2011 Elsevier B.V. All rights reserved.