Imp (IGF-II mRNA-binding protein) is expressed during spermatogenesis in Drosophila melanogaster
Imp (IGF-II mRNA-binding protein) is expressed during spermatogenesis in Drosophila melanogaster
复制标题
DOI：
10.4161/fly.5659
复制
发表时间：
2008-01-01
期刊：
影响因子：
1.2
通讯作者：
Regis, Nadine
中科院分区：
文献类型：
--
作者：
Fabrizio, James J.;Hickey, Christina A.;Regis, Nadine
果蝇精子发生导致单个创始细胞产生 64 个类似于 2 毫米的精子。然而，人们对精子细胞能伸长到如此惊人的长度知之甚少。在对 GFP 标记的蛋白质陷阱集合进行部分筛选时，发现了四个插入片段，它们在精子细胞囊肿的尾端和睾丸的顶端内表现出表达，表明这些蛋白质陷阱可能分别代表与精子细胞伸长和减数分裂前精子发生有关的基因。反向 PCR、随后的循环测序和 BLAST 结果表明，所有四种蛋白质陷阱均代表已知翻译调节因子 Imp（IGF-IImRNA 结合蛋白）内的插入。睾丸增强子陷阱分析还揭示了顶端细胞中的Imp表达，表明Imp在初级精母细胞阶段之前转录。综上所述，这些结果表明 Imp 在精子细胞伸长和减数分裂前精子发生过程中的雄性种系中发挥作用。
Drosophila spermatogenesis results in the production of sixty-four similar to 2-mm spermatozoa from an individual founder cell. Little is known, however, about the elongation of spermatids to such an extraordinary length. In a partial screen of a GFP-tagged protein trap collection, four insertions were uncovered that exhibit expression toward the tail ends of spermatid cysts and within the apical tip of the testis, suggesting that these protein traps may represent genes involved in spermatid elongation and pre-meiotic spermatogenesis, respectively. Inverse PCR followed by cycle sequencing and BLAST revealed that all four protein traps represent insertions within Imp (IGF-IImRNA binding protein), a known translational regulator. Testis enhancer trap analysis also reveals Imp expression in the cells of the apical tip, suggesting transcription of Imp prior to the primary spermatocyte stage. Taken together, these results suggest a role for Imp in the male germline during both spermatid elongation and premeiotic spermatogenesis.