Lack of direct androgen regulation of PDE5 expression.
Lack of direct androgen regulation of PDE5 expression.
复制标题
DOI：
10.1016/j.bbrc.2009.01.144
复制
发表时间：
2009-03-20
影响因子：
3.1
通讯作者：
Lin, Ching-Shwun
中科院分区：
文献类型：
--
作者：
Yang, Rong;Huang, Yun-Ching;Lin, Guiting;Wang, Guifang;Hung, Steven;Dai, Yu-Tian;Sun, Ze-Yu;Lue, Tom F.;Lin, Ching-Shwun
有报道称，阴茎PDE5的表达受雄激素调控。然而，在阉割动物中观察到的PDE5表达变化是受雄激素的直接调节还是由于平滑肌含量的变化，目前尚不清楚。在本研究中，我们发现阉割大鼠引起阴茎大小和海绵状平滑肌含量的减少。免疫染色检测到PDE5和α-平滑肌肌动蛋白（α-SMA）在去势大鼠海绵体中的表达降低。Real-time PCR和western blotting检测去势大鼠PDE5表达与α-SMA表达归一化后无变化。dht处理的大鼠海绵状平滑肌细胞（CSMC）雄激素受体（AR）表达增加，而PDE5表达不变。在dht处理的CSMC中，前列腺特异性抗原（PSA）启动子活性上调，而PDE5A启动子活性保持不变。因此，PDE5的表达不受雄激素的直接调节。
It has been reported that penile PDE5 expression was under androgen regulation. However it remained unknown whether the observed change in PDE5 expression in castrated animals was under direct androgen regulation or due to changes in smooth muscle content. In the present study we showed that castration of rats caused a reduction of penile size and cavernous smooth muscle content. Immunostaining detected concomitant reduction of PDE5 and alpha smooth muscle actin (α-SMA) expression in the corpus cavernosum of castrated rats. Real-time PCR and western blotting detected no change of PDE5 expression when normalized with α–SMA expression in castrated rats. Androgen receptor (AR) expression was increased while PDE5 expression remained unchanged in DHT-treated rat cavernous smooth muscle cells (CSMC). Prostate specific antigen (PSA) promoter activity was upregulated while PDE5A promoter activity remained unchanged in DHT-treated CSMC. Thus, PDE5 expression was not under direct androgen regulation.
登录
查看更多内容
新的雄激素受体基因组靶标显示与ETS1转录因子的相互作用。
影响因子：
7.7
作者：
Massie, Charles E.;Adryan, Boris;Barbosa-Morais, Nuno L.;Lynch, Andy G.;Tran, Maxine G.;Neal, David E.;Mills, Ian G.
通讯作者：
Mills, Ian G.
影响因子：
--
作者：
Burnett, Arthur L.
通讯作者：
Burnett, Arthur L.
影响因子：
4.8
作者：
Morelli, A;Filippi, S;Maggi, M
通讯作者：
Maggi, M
影响因子：
6.6
作者：
Lin, GT;Xin, ZC;Lin, CS
通讯作者：
Lin, CS
影响因子：
6.6
作者：
Banie, Lia;Lin, Guiting;Lin, Ching-Shwun
通讯作者：
Lin, Ching-Shwun