The Genome and Methylome of a Beetle with Complex Social Behavior, Nicrophorus vespilloides (Coleoptera: Silphidae).
The Genome and Methylome of a Beetle with Complex Social Behavior, Nicrophorus vespilloides (Coleoptera: Silphidae).
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DOI：
10.1093/gbe/evv194
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发表时间：
2015-10-09
影响因子：
3.3
通讯作者：
Moore AJ
中科院分区：
文献类型：
--
作者：
Cunningham CB;Ji L;Wiberg RA;Shelton J;McKinney EC;Parker DJ;Meagher RB;Benowitz KM;Roy-Zokan EM;Ritchie MG;Brown SJ;Schmitz RJ;Moore AJ
测试保守的和新的机制表型进化的基础上，需要一个多样性的基因组可用于比较跨越多个独立的谱系。例如，昆虫复杂的社会行为主要是用真社会谱系来研究的，几乎所有的真社会谱系都是膜翅目。如果保守的基因组对社会性的影响确实存在，我们需要来自更广泛的分类群的数据，这些分类群的社会性水平也各不相同。在这里，我们提出了组装和注释的亚社会甲虫Nicrophorus vespilloides，一个物种长期用于调查复杂的社会行为的进化问题的基因组。我们用这个基因组来解决两个问题。首先，生活史的某些方面，例如用尸体繁殖，是否比单基因更能预测基因模型的重叠？我们发现N. vespilloides和所有其他昆虫群体，无论生活史。第二，与其他具有高度社会行为的昆虫一样，但与其他甲虫不同的是，N。vespilloides有DNA甲基化吗？我们发现了活跃的DNA甲基化系统的有力证据。甲基化的分布与具有最多甲基化CpG的外显子的其他昆虫相似。甲基化状态高度保守，N.在膜翅目丽翅蜂中，Vespilloides也被甲基化。这个基因组的增加增加了鞘翅目资源，以回答有关社会性的进化和机制基础的问题，并解决有关甲基化在社会行为中的潜在作用的问题。
Testing for conserved and novel mechanisms underlying phenotypic evolution requires a diversity of genomes available for comparison spanning multiple independent lineages. For example, complex social behavior in insects has been investigated primarily with eusocial lineages, nearly all of which are Hymenoptera. If conserved genomic influences on sociality do exist, we need data from a wider range of taxa that also vary in their levels of sociality. Here, we present the assembled and annotated genome of the subsocial beetle Nicrophorus vespilloides, a species long used to investigate evolutionary questions of complex social behavior. We used this genome to address two questions. First, do aspects of life history, such as using a carcass to breed, predict overlap in gene models more strongly than phylogeny? We found that the overlap in gene models was similar between N. vespilloides and all other insect groups regardless of life history. Second, like other insects with highly developed social behavior but unlike other beetles, does N. vespilloides have DNA methylation? We found strong evidence for an active DNA methylation system. The distribution of methylation was similar to other insects with exons having the most methylated CpGs. Methylation status appears highly conserved; 85% of the methylated genes in N. vespilloides are also methylated in the hymentopteran Nasonia vitripennis. The addition of this genome adds a coleopteran resource to answer questions about the evolution and mechanistic basis of sociality and to address questions about the potential role of methylation in social behavior.