Buffer Standards for the Biochemical pH of 3-(N-morpholino)-2-hydroxypropanesulfonic Acid from (278.15 to 328.15) K.
Buffer Standards for the Biochemical pH of 3-(N-morpholino)-2-hydroxypropanesulfonic Acid from (278.15 to 328.15) K.
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3-(N-吗啉代)-2-羟基丙磺酸生化 pH 值 (278.15 至 328.15) K 的缓冲标准品。
DOI：
10.1021/je800983y
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发表时间：
2009
影响因子：
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通讯作者：
Harmon,MeaganA
中科院分区：
文献类型：
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作者：
Roy,LakshmiN;Roy,RabindraN;Denton,ColeE;Lenoue,SeanR;Roy,ChandraN;Ashkenazi,Shahaf;Fuge,MichaelS;Wollen,JoshuaT;Stegner,JessicaM;Allen,KathleenA;Harmon,MeaganA
在（278.15~328.15）K温度范围内测定了两性电解质3-（N-吗啉代）-2-羟基丙磺酸（MOPSO）的第二离解常数pK2和相关热力学量。在这项研究中，由于贝茨-古根海姆的约定在I = 0.1 mol·kg − 1时有效，因此，在（278.15 - 328.15）K的12个温度下，使用德拜-胡伊克尔方程的扩展形式，评估了两种不含NaCl的缓冲溶液和三种含NaCl且离子强度（I = 0.16 mol·kg − 1）与血浆相似的缓冲溶液的pH值。用流动结池测量了在（298.15和310.15）K时，MOPSO缓冲溶液与甘汞电极饱和KCl溶液之间的液接电位（Ej）。这些Ej值已用于确定（298.15和310.15）K下的操作pH值。推荐三种MOPSO缓冲溶液作为离子强度I = 0.16 mol·kg − 1的盐介质中pH测量的有用参比溶液。
The values of the second dissociation constant pK2and related thermodynamic quantities of the ampholyte 3-(N-morpholino)-2-hydroxypropanesulfonic acid (MOPSO) have been previously determined at temperatures from (278.15 to 328.15) K. In this study, the pH values of two buffer solutions without NaCl and three buffer solutions with NaCl having ionic strengths (I= 0.16 mol·kg−1) similar to those in blood plasma, have been evaluated at 12 temperatures from (278.15 to 328.15) K using an extended form of the Debye−Hückel equation, since the Bates−Guggenheim convention is valid up toI= 0.1 mol·kg−1. The liquid junction potentials (Ej) between the buffer solutions of MOPSO and the saturated KCl solution of the calomel electrode at (298.15 and 310.15) K have been estimated by measurement with a flowing junction cell. These values ofEjhave been used to ascertain the operational pH values at (298.15 and 310.15) K. Three buffer solutions of MOPSO are recommended as useful reference solutions for pH measurements in saline media of ionic strengthI= 0.16 mol·kg−1.