Rapid detection of the Fc gamma RIIA-H/R(131) ligand-binding polymorphism using an allele-specific restriction enzyme digestion (ASRED)
Rapid detection of the Fc gamma RIIA-H/R(131) ligand-binding polymorphism using an allele-specific restriction enzyme digestion (ASRED)
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DOI：
10.1016/s0022-1759(96)00164-0
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发表时间：
1996-11-29
影响因子：
2.2
通讯作者：
McKenzie, SE
中科院分区：
文献类型：
--
作者：
Jiang, XM;Arepally, G;McKenzie, SE
Fc γ RIIA基因的多态性，精氨酸（R）或组氨酸（H）在131位，改变受体结合某些IgG亚类的能力。Fc γ RIIA-H/R（131）基因型的鉴定在宿主防御障碍、免疫血液学疾病和全身性自身免疫性疾病中具有越来越重要的意义。我们报告了一种新的方法，用于确定这种基因型，其中等位基因特异性限制性内切酶位点被引入到Fc γ RIIA PCR产物从基因组DNA，和多态性分配确定限制性内切酶消化，然后琼脂糖凝胶电泳。这种方法比目前可用的方法更快速、更可靠、更便宜。
A polymorphism of the gene for Fc gamma RIIA, arginine (R) or histidine (H) at position 131, alters the ability of the receptor to bind certain IgG subclasses. Identification of the Fc gamma RIIA-H/R(131) genotype has assumed increasing importance in disorders of host defense, immunohematologic diseases and systemic autoimmune disorders. We report a new method for determination of this genotype in which an allele-specific restriction enzyme site is introduced into an Fc gamma RIIA PCR product from genomic DNA, and polymorphism assignment is determined by restriction enzyme digestion followed by agarose gel electrophoresis. This method is more rapid, more reliable and less expensive than currently available methods.