Pulsed-field gel electrophoresis-based subtyping of DNA degradation-sensitive Salmonella enterica subsp enterica serovar Livingstone and serovar Cerro isolates obtained from a chicken layer farm
Pulsed-field gel electrophoresis-based subtyping of DNA degradation-sensitive Salmonella enterica subsp enterica serovar Livingstone and serovar Cerro isolates obtained from a chicken layer farm
复制标题
DOI：
10.1016/j.vetmic.2003.11.014
复制
发表时间：
2004-04-05
影响因子：
3.3
通讯作者：
Otsuki, K
中科院分区：
文献类型：
--
作者：
Murase, T;Nagato, M;Otsuki, K
肠道沙门氏菌血清变种亚种肠道Livingstone和Cerro血清型分离物来自商业产蛋农场，由于PFGE过程中DNA降解，这些分离物以前无法通过脉冲场凝胶电泳（PFGE）分型。使用补充有50 μ M硫脲的电泳缓冲液成功地给出了带型。在硫脲存在下，通过脉冲场凝胶电泳，DNA降解敏感的S。发现来自商业产蛋农场的肠道血清型Cerro分离株与S.肠道血清型Cerro分离株，在不存在硫脲的情况下产生模式。45/50（90%）S。来自农场的肠道血清型Livingstone分离株显示任意指定的XbaI消化模式X1和X2，其通过一条带差异区分，并具有相同的BlnI消化模式。在农场的两个层舍之一。具有模式X2的分离物的数量从1997年的57%增加到1998年的89%，而几乎所有在同一时期从另一个房子获得的分离物都显示模式X1。这表明具有模式X2的菌株可能具有优先在前房屋中定殖的优势。(C)2004 Elsevier B. V.保留所有权利。
Salmonella enterica serovar subsp. enterica Livingstone and serovar Cerro isolates from a commercial egg-producing farm, which had previously been untypeable by pulsed-field gel electrophoresis (PFGE) because of DNA degradation during the PFGE process. successfully gave banding patterns using electrophoresis buffer supplemented with 50 muM thiourea. By PFGE in the presence of thiourea, DNA degradation-sensitive S. enterica serovar Cerro isolates from the commercial egg-producing farm were found to be genetically unrelated to S. enterica serovar Cerro isolates that gave the patterns in the absence of thiourea. Forty-five of 50 (90%) S. enterica serovar Livingstone isolates from the farm showed arbitrarily designated XbaI-digested patterns X1 and X2 that were distinguished by one-band difference and had an identical BlnI-digested pattern. In one of the two layer houses in the farm. the numbers of isolates having the pattern X2 increased from 57% in 1997 to 89% in 1998, whereas virtually all the isolates obtained from the other house in the same period showed the profile X1. This suggests that strains having the pattern X2 might have an advantage to preferentially colonize in the former house. (C) 2004 Elsevier B.V. All rights reserved.