Novosphingobium taihuense sp nov., a novel aromatic-compound-degrading bacterium isolated from Taihu Lake, China
Novosphingobium taihuense sp nov., a novel aromatic-compound-degrading bacterium isolated from Taihu Lake, China
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DOI：
10.1099/ijs.0.63468-0
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发表时间：
2005-05-01
影响因子：
2.8
通讯作者：
Liu, SJ
中科院分区：
文献类型：
--
作者：
Liu, ZP;Wang, BJ;Liu, SJ
从太湖底泥中分离到一株新的芳香族化合物降解菌T3-B 9（T）。该菌株对苯酚、苯胺、硝基苯、4-氯硝基苯和菲等芳香族化合物具有同化作用。采用多相分类法对菌株T3-B 9（T）进行了分类研究。该生物体表现出一系列的表型和化学分类学特性一致的属Novosphingobium。T3-B 9（T）菌株与新鞘氨醇菌属（Novosphingobium）的16 SrRNA基因序列相似性为91.6 ~ 97.5%，与新鞘氨醇菌属（Novosphingobium）的成员聚为一类。菌株T3-B 9（T）与最具遗传学相关性的菌种（亚亚甲基新鞘氨醇菌DSM 12447（T）、食芳香新鞘氨醇菌ATCC 700278（T）和stygium新鞘氨醇菌ATCC 700280（T））的DNA-DNA相关性值分别为31%、33%和14%。结合基因型和表型数据表明，菌株T3-B 9（T）代表新鞘氨醇菌属的一个新种，建议将其命名为太湖新鞘氨醇菌新种（Novosphingobium taihuense sp. nov.）。模式菌株为T3-B 9（T）（=AS 1.3432（T）= JCM 12465（T））。
A novel aromatic-compound-degrading bacterium, strain T3-B9(T), was isolated from sediment of Taihu Lake, Jiangsu Province, south-east China. This bacterial isolate assimilated several aromatic compounds such as phenol, aniline, nitrobenzene, 4-chloronitrobenzene and phenanthrene. The taxonomy of strain T3-B9(T) was studied by polyphasic methods. The organism showed a range of phenotypic and chemotaxonomic properties consistent with those of the genus Novosphingobium. The 16S rRNA gene sequence similarity of strain T3-B9(T) to members of the genus Novosphingobium ranged from 91.6 to 97.5%, and this isolate clustered phylogenetically with members of genus Novosphingobium. The DNA-DNA relatedness values of strain T3-B9(T) to the most phylogenetically related species, Novosphingobium subterraneum DSM 12447(T), Novosphingobium aromaticivorans ATCC 700278(T) and Novosphingobium stygium ATCC 700280(T), were 31, 33 and 14%, respectively. The combined genotypic and phenotypic data show that strain T3-B9(T) represents a novel species of the genus Novosphingobium, for which the name Novosphingobium taihuense sp. nov. is proposed. The type strain is T3-B9(T) (=AS 1.3432(T) = JCM 12465(T)).