Localization and phosphorylation of Ab1-interactor proteins, Abi-1 and Abi-2, in the developing nervous system
Localization and phosphorylation of Ab1-interactor proteins, Abi-1 and Abi-2, in the developing nervous system
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DOI：
10.1006/mcne.2000.0865
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发表时间：
2000-09-01
影响因子：
3.5
通讯作者：
Pendergast, AM
中科院分区：
文献类型：
--
作者：
Courtney, KD;Grove, M;Pendergast, AM
β-相互作用蛋白（Abi）是β-家族非受体酪氨酸激酶的靶蛋白，并且是响应于生长因子刺激的Rac依赖性细胞骨架重组所必需的。我们询问Abi-1和Abi-2的表达、磷酸化和细胞定位是否支持这些蛋白在发育和成年小鼠神经系统中Abl信号传导中的作用。在妊娠中期至晚期胚胎中，abi-2信息在中枢和外周神经系统（CNS和PNS）中升高。Abi-1 mRNA在CNS中存在，但不增强，并且在PNS结构中未观察到。来自脑裂解物的Abi蛋白质随着年龄的增加而经历表观分子量和磷酸化的变化。在出生后的大脑中，abi-1和abi-2在投射神经元聚集的皮质层中表达最显著。在培养的神经元中，Abi-1和Abi-2集中在贯穿细胞体和突起的斑点中。Abi和Abl蛋白都存在于突触体和生长锥颗粒中。因此，Abi衔接子表现出适当的表达模式和亚细胞定位，以参与神经系统中的Abl激酶信号传导。
Abl-interactor (Abi) proteins are targets of Abl-family nonreceptor tyrosine kinases and are required for Rac-dependent cytoskeletal reorganization in response to growth factor stimulation. We asked if the expression, phosphorylation, and cellular localization of Abi-1 and Abi-2 supports a role for these proteins in Abl signaling in the developing and adult mouse nervous system. In mid- to late-gestation embryos, abi-2 message is elevated in the central and peripheral nervous systems (CNS and PNS). Abi-1 mRNA is present, but not enhanced, in the CNS, and is not observed in PNS structures. Abi proteins from brain lysates undergo changes in apparent molecular weight and phosphorylation with increasing age. In the postnatal brain, abi-1 and abi-2 are expressed most prominently in cortical layers populated by projection neurons. In cultured neurons, Abi-1 and Abi-2 are concentrated in puncta throughout the cell body and processes. Both Abi and Abl proteins are present in synaptosomes and growth cone particles. Therefore, the Abi adaptors exhibit proper expression patterns and subcellular localization to participate in Abl kinase signaling in the nervous system.