Anti-LRP/LR-Specific Antibody IgG1-iS18 Significantly Reduces Adhesion and Invasion of Metastatic Lung, Cervix, Colon and Prostate Cancer Cells
Anti-LRP/LR-Specific Antibody IgG1-iS18 Significantly Reduces Adhesion and Invasion of Metastatic Lung, Cervix, Colon and Prostate Cancer Cells
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DOI：
10.1016/j.jmb.2012.02.035
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发表时间：
2012-05-25
影响因子：
5.6
通讯作者：
Weiss, Stefan F. T.
中科院分区：
文献类型：
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作者：
Omar, Aadilah;Reusch, Uwe;Weiss, Stefan F. T.
37-kDa/67-kDa层粘连蛋白受体[层粘连蛋白受体前体/高亲和力层粘连蛋白受体（LRP/LR）]被认为在转移性癌症的关键组分侵袭和粘附中起主要作用。肺癌、宫颈癌、结肠癌和前列腺癌是全球十大癌症类型之一。在这里，我们报告了肺癌细胞，宫颈癌细胞，结肠癌细胞和前列腺癌细胞表面的LRP/LR水平与非致瘤性成纤维细胞相比显著增加。使用抗LRP/LR特异性抗体IgG 1-iS 18，肺癌细胞、宫颈癌细胞、结肠癌细胞和前列腺癌细胞与层粘连蛋白-1的粘附显著降低。IgG 1-iS 18显著阻碍这些细胞系侵入Matrigel（TM）基质。Pearson相关系数证明了LRP/LR细胞表面水平与侵袭潜力以及粘附和侵袭之间的相关性。我们的研究结果表明，IgG 1-iS 18抗体可能作为治疗各种转移性癌症类型的替代治疗工具。(C)2012爱思唯尔有限公司版权所有。
The 37-kDa/67-kDa laminin receptor [laminin receptor precursor/high-affinity laminin receptor (LRP/LR)] is thought to play a major role in invasion and adhesion, key components of metastatic cancer. Lung cancer, cervical cancer, colon cancer and prostate cancer are among the top 10 cancer types worldwide. Here, we report that LRP/LR levels on the surface of lung cancer cells, cervical cancer cells, colon cancer cells and prostate cancer cells are significantly increased compared to non-tumorigenic fibroblasts. Adhesion of lung cancer cells, cervical cancer cells, colon cancer cells and prostate cancer cells to laminin-1 is significantly reduced, employing the anti-LRP/LR-specific antibody IgG1-iS18. Invasion of these cell lines into the Matrigel (TM) matrix was significantly impeded with IgG1-iS18. The Pearson's correlation coefficient proves a correlation between LRP/LR cell-surface levels and invasion potential, as well as adhesion and invasion, respectively. Our findings suggest that IgG1-iS18 antibody might act as alternative therapeutic tool for treatment of various metastatic cancer types. (C) 2012 Elsevier Ltd. All rights reserved.