Decreased thymosin β4 in apoptosis induced by a variety of antitumor drugs
Decreased thymosin β4 in apoptosis induced by a variety of antitumor drugs
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DOI：
10.1016/s0006-2952(99)00030-1
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发表时间：
1999-05-15
影响因子：
5.8
通讯作者：
Ishida, R
中科院分区：
文献类型：
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作者：
Iguchi, K;Usami, Y;Ishida, R
由于许多抗肿瘤药物可以通过诱导细胞凋亡来杀死肿瘤，如果这些药物与其他与细胞凋亡过程相互作用的药物联合使用，可能会增强这些药物的作用。为了阐明诱导细胞凋亡所涉及的生物学事件，我们检测了抗肿瘤药物诱导细胞凋亡相关蛋白的变化。当Molt-4细胞暴露于抗肿瘤药物依托oposide， meso-2,3-bis（3,5- dioxop哌嗪-1-yl）丁烷（ICRF-193）和neocarzinostatin时，它们表现出凋亡细胞死亡，通过流式细胞术使用异硫氰酸荧光素（FITC）标记的膜联蛋白V染色膜上的磷脂酰丝氨酸和检测次二倍体细胞。诱导细胞凋亡后，一种低分子量蛋白（经HPLC分析鉴定为胸腺肽β(4)）普遍减少，肌动蛋白丝形态变为团块状。这些结果表明，胸腺酶β(4)的降低参与了抗肿瘤药物诱导细胞凋亡的过程。(C) 1999 Elsevier Science Inc.；
As many antitumor drugs can kill tumors through the induction of apoptosis, the effect of these drugs presumably would be enhanced ii they were used in combination with other drugs that interact with apoptotic processes. To clarify the biological events involved in the induction of apoptosis, we examined changes in the proteins associated with induction of apoptosis by antitumor drugs. When Molt-4 cells were exposed to the antitumor drugs etoposide, meso-2,3-bis(3,5-dioxopiperazine-1-yl)butane (ICRF-193), and neocarzinostatin, they exhibited apoptotic cell death as determined by flow cytometry using fluorescein isothiocyanate (FITC)-labeled annexin V staining of phosphatidylserine on membranes and detection of hypodiploid cells. Following the induction of apoptosis, a low molecular weight protein that was identified to be thymosin beta(4) by HPLC analysis was commonly decreased, and the morphology of actin filaments changed into clump formations. These results suggest that decreased thymosin beta(4) is involved in the induction of apoptosis hy antitumor drugs. (C) 1999 Elsevier Science Inc.