RhoA and RhoC are involved in stromal cell-derived factor-1-induced cell migration by regulating F-actin redistribution and assembly
RhoA and RhoC are involved in stromal cell-derived factor-1-induced cell migration by regulating F-actin redistribution and assembly
复制标题
RhoA 和 RhoC 通过调节 F-肌动蛋白重新分布和组装参与基质细胞衍生因子 1 诱导的细胞迁移
DOI：
10.1007/s11010-017-3072-3
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发表时间：
2017-12-01
影响因子：
4.3
通讯作者：
Zeng, Xianlu
中科院分区：
文献类型：
--
作者：
Luo, Jixian;Li, Dingyun;Zeng, Xianlu
基质细胞衍生因子-1（SDF-1）信号通过诱导趋化性迁移在T细胞急性淋巴细胞白血病的维持和进展中起重要作用。为了鉴定SDF-1信号传导在T-ALL迁移中的机制，使用Jurkat急性淋巴细胞白血病细胞。结果表明，SDF-1通过F-actin重新分布和组装诱导Jurkat细胞迁移，这依赖于Rho活性。SDF-1诱导RhoA和RhoC活化，以及活性氧（ROS）的产生，这是由Rho抑制剂抑制。在迁移过程中，依赖于Rho的ROS产生导致随后的细胞骨架重新分布和组装。此外，RhoA和RhoC参与SDF-1诱导的Jurkat细胞迁移。总而言之，我们发现了SDF-1/CXCR 4-RhoA和RhoC-ROS-细胞骨架途径，可以调节Jurkat细胞响应SDF-1的迁移。这一工作将有助于更清楚地了解急性淋巴细胞白血病的迁移机制。
Stromal cell-derived factor-1 (SDF-1) signaling is important to the maintenance and progression of T-cell acute lymphoblastic leukemia by inducing chemotaxis migration. To identify the mechanism of SDF-1 signaling in the migration of T-ALL, Jurkat acute lymphoblastic leukemia cells were used. Results showed that SDF-1 induces Jurkat cell migration by F-actin redistribution and assembly, which is dependent on Rho activity. SDF-1 induced RhoA and RhoC activation, as well as reactive oxygen species (ROS) production, which was inhibited by Rho inhibitor. The Rho-dependent ROS production led to subsequent cytoskeleton redistribution and assembly in the process of migration. Additionally, RhoA and RhoC were involved in SDF-1-induced Jurkat cell migration. Taken together, we found a SDF-1/CXCR4-RhoA and RhoC-ROS-cytoskeleton pathway that regulates Jurkat cell migration in response to SDF-1. This work will contribute to a clearer insight into the migration mechanism of acute lymphoblastic leukemia.