Effect of green tea extract on reactive oxygen species produced by neutrophils from cancer patients.
Effect of green tea extract on reactive oxygen species produced by neutrophils from cancer patients.
复制标题
绿茶提取物对癌症患者中性粒细胞产生的活性氧的影响。
作者：
Katsuhiko Suzuki;S. Ohno;Yoko Suzuki;Y. Ohno;Ryuji Okuyama;A. Aruga;Masakazu Yamamoto;Ken;Tsutomu Nozaki;S. Miura;H. Yoshioka;Y. Mori
背景/目标
癌症患者的氧化应激已被证明部分是由中性粒细胞介导的。尽管据报道绿茶提取物等天然抗氧化剂可以减少氧化应激，但其对癌症患者的作用证据有限。本研究旨在确定绿茶提取物对癌症患者中性粒细胞产生的活性氧的影响。
材料和方法
外周血样本取自 18 名晚期癌症患者。将绿茶提取物与 Mebiol 凝胶上的鲁米诺一起添加到血样中，并测量鲁米诺依赖性化学发光，以监测迁移到凝胶中的中性粒细胞在 37°C 下产生的活性氧。
结果
在绿茶提取物存在下，鲁米诺依赖性化学发光以浓度依赖性方式显着下调。
结论
这些结果表明绿茶提取物对中性粒细胞产生的活性氧具有抗氧化作用，这可能有效减少癌症患者的氧化应激。
BACKGROUND/AIM
Oxidative stress in cancer patients has been demonstrated to be partly mediated by neutrophils. Although it is reported that natural antioxidants, such as green tea extract, reduce oxidative stress, there is limited evidence of their effects in cancer patients. This study aimed to determine the effect of green tea extract on reactive oxygen species produced by neutrophils from cancer patients.
MATERIALS AND METHODS
Peripheral blood samples were obtained from eighteen patients with advanced cancer. Green tea extract was added to the blood samples with luminol on Mebiol gel, and luminol-dependent chemiluminescence was measured to monitor the production of reactive oxygen species from migrated neutrophils into the gel, at 37°C.
RESULTS
Luminol-dependent chemiluminescence was significantly down-regulated in the presence of green tea extract in a concentration-dependent manner.
CONCLUSION
These results indicate the antioxidant effect of green tea extract on reactive oxygen species produced by neutrophils, which may be effective in reducing oxidative stress in cancer patients.
影响因子：
5.6
作者：
Zhu J;Wang O;Ruan L;Hou X;Cui Y;Wang JM;Le Y
通讯作者：
Le Y
DOI：
10.1016/j.bbrc.2011.09.115
发表时间：
2011-10-28
影响因子：
3.1
作者：
Ellis, Lixia Z.;Liu, Weimin;Luo, Yuchun;Okamoto, Miyako;Qu, Dovina;Dunn, Jeffrey H.;Fujita, Mayumi
通讯作者：
Fujita, Mayumi