Influence of ionic crosslinkers (Ca2+/Ba2+/Zn2+) on the mechanical and biological properties of 3D Bioplotted Hydrogel Scaffolds
Influence of ionic crosslinkers (Ca2+/Ba2+/Zn2+) on the mechanical and biological properties of 3D Bioplotted Hydrogel Scaffolds
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DOI：
10.1080/09205063.2018.1433420
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发表时间：
2018-01-01
影响因子：
3.6
通讯作者：
Chen, Xiongbiao
中科院分区：
文献类型：
--
作者：
Sarker, Md.;Izadifar, Mohammad;Chen, Xiongbiao
三维（3D）生物绘图需要适当的交联剂来交联水凝胶前体，同时保持包埋细胞的活力。然而，各种类型的交联剂在生物绘图的评价和比较仍然underexplored日期。本文研究了三种离子交联剂氯化钙（CaCl 2）、氯化钡（BaCl 2）和氯化锌（ZnCl 2）对三维生物绘图藻酸盐支架的力学和生物学性能的影响。支架的机械性能包括弹性模量，溶胀和降解，而考虑的生物学特性包括雪旺细胞活力和表面形态。生物绘图支架的机械和生物学性质都取决于用于制造的交联剂;具体地，交联离子导致水凝胶的弹性模量在42天内以BaCl 2> CaCl 2> ZnCl 2的顺序降低，而施万细胞活力在7天内以CaCl 2> BaCl 2> ZnCl 2的顺序降低。总之，这些结果提供了在操纵3D生物绘图过程方面有效的见解，以便调整和优化用于组织工程应用的所绘制支架的机械和生物性能。
Three dimensional (3D) bioplotting requires appropriate crosslinkers to crosslink the hydrogel precursor while simultaneously maintaining the viability of embedded cells. However, the evaluation and comparison of various types of crosslinkers in bioplotting remains underexplored to date. This paper presents our study of the influence of three ionic crosslinkerscalcium chloride (CaCl2), barium chloride (BaCl2), and zinc chloride (ZnCl2)on the mechanical and biological properties of 3D bioplotted alginate scaffolds. The scaffold mechanical properties characterized included the elastic modulus, swelling, and degradation while the biological properties considered included Schwann cell viability and surface morphology. The mechanical and biological properties of the bioplotted scaffolds were both dependent on the crosslinkers used for fabrication; specifically, crosslinking ions resulted in the elastic modulus of the hydrogels decreasing in the order BaCl2>CaCl2>ZnCl2 over 42days while Schwann cell viability decreased in the order CaCl2>BaCl2>ZnCl2 over 7days. Taken together, these results offer insights that are effective in terms of manipulating the 3D bioplotting process so as to tune and optimize the mechanical and biological performance of the plotted scaffolds for tissue engineering applications.