Abnormal spindle protein, asp, and the integrity of mitotic centrosomal microtubule organizing centers
Abnormal spindle protein, asp, and the integrity of mitotic centrosomal microtubule organizing centers
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DOI：
10.1126/science.283.5408.1733
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发表时间：
1999-03-12
期刊：
影响因子：
56.9
通讯作者：
Glover, DM
中科院分区：
文献类型：
--
作者：
Avides, MD;Glover, DM
异常纺锤体(Asp)基因的产物被发现是有丝分裂过程中中心体的不对称定位成分，需要在体内集中有丝分裂纺锤体的两极。从果蝇胚胎提取物中去除Asp蛋白的功能，无论是通过突变还是免疫耗竭，都会导致它们失去将微管组织中心恢复到盐剥离的中心体制剂中的能力。这一点通过添加纯化的天冬氨酸蛋白而得到纠正。因此，Asp似乎将构成有丝分裂中心体的微管核伽马微管蛋白环复合体结合在一起。
The product of the abnormal spindle (asp) gene was found to be an asymmetrically Localized component of the centrosome during mitosis, required to focus the poles of the mitotic spindle in vivo. Removing Asp protein function from Drosophila melanogaster embryo extracts, either by mutation or immunodepletion, resulted in Loss of their ability to restore microtubule-organizing center activity to salt-stripped centrosome preparations. This was corrected by addition of purified Asp protein. Thus, Asp appears to hold together the microtubule-nucleating gamma-tubulin ring complexes that organize the mitotic centrosome.