Evaluation of the light-sensitive cytotoxicity of Hypericum perforatum extracts, fractions, and pure compounds
Evaluation of the light-sensitive cytotoxicity of Hypericum perforatum extracts, fractions, and pure compounds
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DOI：
10.1021/jf052344k
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发表时间：
2006-04-19
影响因子：
6.1
通讯作者：
Birt, DF
中科院分区：
文献类型：
--
作者：
Schmitt, LA;Yi, L;Birt, DF
贯叶连翘（Hypericum perforatum，Hp）具有抗抑郁和抗病毒活性。尽管它被用作传统抗抑郁药的替代品，但对这种草药的细胞毒性化学物质的鉴定是不完整的。在本研究中，在三种细胞系中检查了在极性范围内的溶剂中制备的髋关节浸提液、一种浸提液的组分和纯化化合物的细胞毒性。所有浸提液均表现出明显的细胞毒性;在乙醇中制备的那些（无贯叶金丝桃素、3.6 μ M金丝桃素和134.6 μ M黄酮类化合物）显示7.7 - 77.4%的细胞存活率（p < 0.0001和0.01），而氯仿和己烷提取物（金丝桃素、金丝桃素和类黄酮未检测到）显示约9.0%（p < 0.0001）和4.0%（p < 0.0001）的存活率。在去除氯仿或己烷中浸提的材料后，主要观察到依次浸提的乙醇浸提液的光敏毒性。Hp提取物不存在光敏毒性，表明金丝桃素在提取物的毒性中没有发挥突出的作用。
Hypericum perforatum (Hp) is known for possessing antidepressant and antiviral activities. Despite its use as an alternative to conventional antidepressants, the identification of the cytotoxic chemicals derived from this herb is incomplete. In this study, the cytotoxicity of Hip extracts prepared in solvents ranging in polarity, fractions of one extract, and purified compounds were examined in three cell lines. All extracts exhibited significant cytotoxicity; those prepared in ethanol (no hyperforin, 3.6 mu M hypericin, and 134.6 mu M flavonoids) showed between 7.7 and 77.4% cell survival (p < 0.0001 and 0.01), whereas the chloroform and hexane extracts (hyperforin, hypericin, and flavonoids not detected) showed approximately 9.0 (p < 0.0001) and 4.0% (p < 0.0001) survival. Light-sensitive toxicity was observed primarily with the ethanol extracts sequentially extracted following removal of material extracted in either chloroform or hexane. The absence of light-sensitive toxicity with the Hp extracts suggests that the hypericins were not playing a prominent role in the toxicity of the extracts.