Fluorimetric monitoring of vancomycin using an allosteric probe-initiated sensing platform.
Fluorimetric monitoring of vancomycin using an allosteric probe-initiated sensing platform.
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DOI：
10.1016/j.aca.2023.341431
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发表时间：
2023-05
影响因子：
6.2
通讯作者：
Jinshan Yuan;G. Xie;Junjie Li;Yaxing Xie;Yuan Yao;Jianhong Zhang;Yulei Hou;Hui Chen
中科院分区：
文献类型：
--
作者：
Jinshan Yuan;G. Xie;Junjie Li;Yaxing Xie;Yuan Yao;Jianhong Zhang;Yulei Hou;Hui Chen
万古霉素是治疗耐甲氧西林金黄色葡萄球菌（MRSA）和多重耐药菌感染的一线药物。万古霉素的有效治疗浓度范围较窄，因此实施万古霉素治疗药物监测至关重要。然而，传统的检测方法存在设备昂贵、操作复杂、重现性差等缺点。在此，构建了由变构探针启动的荧光传感平台，用于以低成本简单、灵敏地监测万古霉素。该平台的关键点是精心设计的变构探针，它由适体和触发序列组成。当万古霉素存在时，万古霉素与适配体的结合会导致变构探针的构象变化，从而暴露触发序列。触发器可以与分子信标（MB）反应产生荧光信号。此外，采用变构探针结合杂交链式反应（HCR）开发了扩增平台，线性范围为0.5μgmL−1至50μgmL−1，检测限（LOD）为0.26μgmL−1。最重要的是，这种变构探针启动的传感平台在人血清样品中表现出良好的检测能力，并且与 HPLC 相比，它还显示出良好的相关性和准确性。目前简单、灵敏的变构探针启动平台有可能支持万古霉素的治疗药物监测，对促进临床合理使用抗生素具有重要意义。
Vancomycin is the first-line drug for infections of methicillin-resistantStaphylococcus aureus(MRSA) and multi-drug-resistant bacteria. The effective therapeutic concentration range of vancomycin is narrow, so it's essential to implement vancomycin therapeutic drug monitoring. However, conventional detection methods have disadvantages of expensive equipment, complicated operation, or poor reproducibility. Herein, a fluorescent sensing platform initiated by an allosteric probe was constructed for simple and sensitive monitoring of vancomycin at a low cost. The key point of this platform is the well-designed allosteric probe, which comprises an aptamer and a trigger sequence. When vancomycin exists, the combination of vancomycin and the aptamer will lead to a conformational change of the allosteric probe, thus exposing the trigger sequence. The trigger can react with the molecular beacon (MB) to generate fluorescent signals. In addition, the allosteric probe combined with hybridization chain reaction (HCR) was applied to develop an amplified platform, the linear range is from 0.5 μg mL−1to 50 μg mL−1with the limit of detection (LOD) of 0.26 μg mL−1. Most importantly, this allosteric probe-initiated sensing platform shows good detection ability in human serum samples, and it also indicates great correlation and accuracy compared with HPLC. The present simple and sensitive allosteric probe-initiated platform has the potential to support the therapeutic drug monitoring of vancomycin, which is of great significance to promote the rational use of antibiotics in clinics.