AuNPs/NiFe-LDHs-assisted laser desorption/ionization mass spectrometry for efficient analysis of metronidazole and its metabolites in water samples
AuNPs/NiFe-LDHs-assisted laser desorption/ionization mass spectrometry for efficient analysis of metronidazole and its metabolites in water samples
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DOI：
10.1016/j.jhazmat.2021.126893
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发表时间：
2021-09-01
影响因子：
13.6
通讯作者：
Ma, Chunxia
中科院分区：
文献类型：
--
作者：
Li, Lingyu;Qiu, Zhichang;Ma, Chunxia
金纳米粒子(AuNPs)作为激光解吸/电离质谱仪(LDI-MS)的纳米材料已被广泛应用于小分子样品的分析。镍/铁层状双氢氧化物(NiFe-LDHs)纳米片可以支持AuNPs的锚定，增强其解吸/电离能力。它们的混杂纳米复合材料有望产生协同效应来改善LDI-MS的性能。本工作采用自组装法制备了一种新型的AuNPs/NiFe-LDHs纳米材料，并用透射电子显微镜、扫描电子显微镜、XPS、UV-Vis和FTIR-ATR对其进行了表征。与传统有机基质相比，AuNPs/NiFe-LDHs辅助LDI-TOF MS具有更高的峰强度和更低的背景噪声。此外，MNZ及其代谢物的检测范围为1-50 ng·m-1(R-2>；0.98)，灵敏度和检出限分别为0.5ng·m·L~(-1)和1 ng·m·L~(-1)，具有良好的耐盐性和蛋白质耐受性、良好的重复性和定量测定能力。这种纳米复合材料还可以用于其他一些小分子的分析，如抗生素、糖、氨基酸和杀虫剂，展示了检测各种环境化学物质的潜力。综上所述，该方法结合了两种纳米材料的优点，可以快速准确地分析水样中的MNZ及其代谢物以及其他一些小分子。
Gold nanoparticles (AuNPs) have been widely used as laser desorption/ionization mass spectrometry (LDI-MS) nanomaterials for the analysis of low-molecular-weight samples. Nickel/iron-layered double hydroxides (NiFe-LDHs) nanosheets can support the anchoring of AuNPs and enhance the ability of desorption/ionization. Their hybrid nanocomposites are expected to produce synergistic effects to improve the performance of LDI-MS. In this work, a novel AuNPs/NiFe-LDHs nanomaterial was synthesized by self-assembly method and characterized based on TEM, SEM, XPS, UV-vis and FTIR-ATR. AuNPs/NiFe-LDHs assisted LDI-TOF MS exhibited higher peak intensity and lower background noise compared with conventional organic matrices. Furthermore, excellent salt and protein tolerance, good repeatability and quantification were observed when MNZ and its metabolites were detected in the range of 1-50 ng.mu L-1 (R-2 > 0.98), with LODs and LOQs of 0.5 ng.mu L-1 and 1 ng.mu L-1, respectively. This nanocomposite could also be used for the analysis of some other small molecules, such as antibiotics, sugars, amino acids and pesticides, demonstrating the potential to detect a variety of environmental chemicals. Taken together, the developed method combined the advantages of two nanomaterials and can provide rapid and accurate analysis of MNZ and its metabolites in water samples, as well as some other small molecules.