Transcranial electrical stimulation of cortico-cortical connections in anesthetized mice
Transcranial electrical stimulation of cortico-cortical connections in anesthetized mice
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DOI：
10.1016/j.jneumeth.2011.08.007
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发表时间：
2011-10-15
影响因子：
3
通讯作者：
Shibuki, Katsuei
中科院分区：
文献类型：
--
作者：
Hishida, Ryuichi;Watanabe, Kenji;Shibuki, Katsuei
我们开发了一种经颅电刺激（TES）技术，以研究小鼠的皮质-皮质连接。在用牙科棒的刀片剃掉头骨之后，将针的钝尖轻轻地推到变薄的头骨上。力使颅骨变形，针尖周围颅骨和皮质之间的蛛网膜下腔最小化。在这些条件下，施加到针上的刺激电流通过变薄的头骨直接流入大脑皮层。通过经颅黄素蛋白荧光成像可视化通过这种方法刺激的皮质-皮质功能连接。TES诱发的皮层反应表现出的空间和时间的活动模式相媲美的传统方法，其中电极直接插入到表层皮层引起的。两种方法的比较表明，TES需要稍强的刺激强度和优先激活皮层的表层相比，传统的方法。使用新的方法，我们揭示了小鼠高级视觉皮质的外侧和内侧部分之间存在相互的皮质-皮质功能连接。这种新方法结合经颅黄素蛋白荧光成像，使我们能够激活皮质-皮质通路产生的初级感觉区和调查感觉信息流在小鼠大脑皮层。(C)2011 Elsevier B. V.保留所有权利。
We developed a technique of transcranial electrical stimulation (TES) to investigate cortico-cortical connections in mice. After the skull was shaved with the blade of a dental bar, a blunt tip of a needle was gently pushed onto the thinned skull. The skull was deformed by the force, and the subarachnoid space between the skull and the cortex was minimized around the needle tip. Under these conditions, stimulus currents applied to the needle directly flowed into the cortex through the thinned skull. Cortico-cortical functional connections stimulated by this method were visualized by transcranial flavoprotein fluorescence imaging. The cortical responses evoked by TES exhibited spatial and temporal activity patterns comparable to those elicited by a conventional method, in which an electrode is directly inserted into superficial cortical layers. A comparison of the two methods revealed that TES required a slightly stronger stimulus intensity and preferentially activated superficial layers of the cortex compared with the conventional method. Using the new method, we revealed the presence of reciprocal cortico-cortical functional connections between lateral and medial parts of higher visual cortices in mice. This new method combined with transcranial flavoprotein fluorescence imaging allowed us to activate cortico-cortical pathways arising from the primary sensory areas and investigate sensory information flow in the mouse cerebral cortex. (C) 2011 Elsevier B.V. All rights reserved.