Advances in proteomics for production strain analysis.
Advances in proteomics for production strain analysis.
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DOI：
10.1016/j.copbio.2015.05.001
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发表时间：
2015-12
影响因子：
7.7
通讯作者：
Andrew Landels;C. Evans;J. Noirel;P. Wright
中科院分区：
文献类型：
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作者：
Andrew Landels;C. Evans;J. Noirel;P. Wright
蛋白质组学在生产菌株分析中有着广泛的应用。通过案例分析，讨论了具体策略的价值。通常基于对真核系统的先前应用的方法学。新的发展目标是定量准确性和蛋白质组覆盖率。蛋白质组学是对蛋白质的大规模研究和分析，旨在分析细胞背景下的蛋白质功能。由于在活细胞中发生的绝大多数过程依赖于蛋白质活性，蛋白质组学提供了一个独特的优势，研究人员可以从中解剖，表征，理解和操纵生物系统。在开发生产菌株时，蛋白质组学提供了一个多功能的分析技术工具包。在这篇评论中，我们通过三个与工业相关的案例研究，重点介绍了该领域的一些最新进展：大肠杆菌异源途径的靶向蛋白质组学分析，聚胞菌PCC6803的生物燃料生产和木质纤维素降解的蛋白质组学研究。最后，我们讨论了蛋白质组学的未来发展，这将对生物生产菌株的代谢工程和过程监测产生影响。
HighlightsProteomics is widely used in production strain analysis.The value of specific strategies is discussed with reference to case studies.Methodologies often based on prior application to eukaryotic systems.New developments target quantitative accuracy and proteome coverage.Proteomics is the large-scale study and analysis of proteins, directed to analysing protein function in a cellular context. Since the vast majority of the processes occurring in a living cell rely on protein activity, proteomics offer a unique vantage point from which researchers can dissect, characterise, understand and manipulate biological systems. When developing a production strain, proteomics offers a versatile toolkit of analytical techniques. In this commentary, we highlight a number of recent developments in this field using three industrially relevant case studies: targeted proteomic analysis of heterologous pathways in Escherichia coli, biofuel production in Synechocystis PCC6803 and proteomic investigations of lignocellulose degradation. We conclude by discussing future developments in proteomics that will impact upon metabolic engineering and process monitoring of bio-producer strains.