Localization of plasmid loci necessary for the entry of Shigella flexneri into HeLa cells, and characterization of one locus encoding four immunogenic polypeptides.
Localization of plasmid loci necessary for the entry of Shigella flexneri into HeLa cells, and characterization of one locus encoding four immunogenic polypeptides.
复制标题
福氏志贺氏菌进入 HeLa 细胞所需的质粒基因座的定位，以及编码四种免疫原性多肽的一个基因座的表征。
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
P. Sansonetti
中科院分区：
文献类型：
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作者：
B. Baudry;A. Maurelli;Philippe Clerc;J. Sadoff;P. Sansonetti
We have previously cloned a 44 kb fragment from the virulence plasmid of Shigella flexneri serotype 5 strain M90T which is capable of restoring invasiveness to an avirulent, plasmidless mutant. This report presents a genetic and physical analysis of Tn5 mutations in recombinant clone pHS4108. Tn5 mutagenesis allowed identification of at least five regions implicated in the entry phenotype. These regions were located on a 20 kb portion of pHS4108. Expression of the insertion mutants was studied by immunoblots using the serum of a convalescent monkey infected by S. flexneri 2a, which recognized four plasmid-associated polypeptides. We propose that the four immunogenic polypeptides, a, b, c, and d, are encoded an operon.
