Ionic liquids as matrixes for matrix-assisted laser desorption/ionization mass spectrometry
Ionic liquids as matrixes for matrix-assisted laser desorption/ionization mass spectrometry
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DOI：
10.1021/ac010259f
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发表时间：
2001-08-01
影响因子：
7.4
通讯作者：
Gross, ML
中科院分区：
文献类型：
--
作者：
Armstrong, DW;Zhang, LK;Gross, ML
室温离子液体作为溶剂用于有机合成、电化学研究和分离。我们希望研究它们的高溶解能力、可忽略的蒸气压和宽的液体温度范围是否是有利的，如果它们用作UV-MALDI的基质。使用肽、蛋白质和聚（乙二醇）（PEG-2000）合成并测试了几种不同的离子基质。与其他常用的液体和固体基质相比，所有测试的离子液体都具有优异的增溶性能和真空稳定性。然而，它们在产生分析物气相离子的能力方面差异很大。然而，某些离子基质产生比目前使用的固体基质具有更高真空稳定性、更高离子峰强度和相等或更低检测限的均匀溶液。显然，离子液体及其更无定形的固体类似物作为MALDI基质值得进一步研究。
Room-temperature ionic liquids are useful as solvents for organic synthesis, electrochemical studies, and separations. We wished to examine whether their high solubalizing power, negligible vapor pressure, and broad liquid temperature range are advantageous if they are used as matrixes for UV-MALDI. Several different ionic matrixes were synthesized and tested, using peptides, proteins, and poly(ethylene glycol) (PEG-2000). All ionic liquids tested have excellent solubilizing properties and vacuum stability compared to other commonly used liquid and solid matrixes. However, they varied widely in their ability to produce analyte gas-phase ions. Certain ionic matrixes, however, produce homogeneous solutions of greater vacuum stability, higher ion peak intensity, and equivalent or lower detection limits than currently used solid matrixes. Clearly, ionic liquids and their more amorphous solid analogues merit further investigation as MALDI matrixes.