Soil spore bank communities of ectomycorrhizal fungi in Pseudotsuga japonica forests and neighboring plantations
Soil spore bank communities of ectomycorrhizal fungi in Pseudotsuga japonica forests and neighboring plantations
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DOI：
10.1007/s00572-021-01065-y
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发表时间：
2022-01
期刊：
影响因子：
3.9
通讯作者：
K. Okada;Y. Matsuda
中科院分区：
文献类型：
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作者：
K. Okada;Y. Matsuda
外生菌根（EcM）真菌孢子在苗木建立和森林更新中起着重要作用，特别是在缺乏相容寄主树种的地区。然而，与其他分布广泛的松科树木相比，濒危植物黄杉与外生菌根真菌之间的相互作用，特别是孢子库的研究却非常有限。本研究的目的是调查土壤中的EcM真菌孢子库群落在日本花旗松（日本黄杉）林残留斑块。我们进行了生物测定的178个土壤样品，从三个P。japonicforests及其邻近的丛枝菌根人工种植园，使用更容易获得的北美花旗松（黄杉menziesii）作为诱饵苗。通过ITS区的形态分型和DNA测序的组合来鉴定EcM真菌物种。我们发现，EcM真菌孢子库不仅存在于P。japonicforests，但也在邻近的种植园。在检测到的13种EcM真菌中，Roppogon togasawarius的频率第二高，在所有样地中均发现，无论森林类型如何。物种丰富度估计值之间的森林类型显着差异。EcM真菌孢子库的群落结构在研究地点之间存在显著差异，但在森林类型之间没有显著差异。这些结果表明，EcM真菌孢子银行不仅限于EcM森林，并延伸到周围的森林为主的丛枝菌根树木，可能是由于EcM真菌孢子在土壤中的耐久性。
Ectomycorrhizal (EcM) fungal spores play an important role in seedling establishment and forest regeneration, especially in areas where compatible host tree species are absent. However, compared to other Pinaceae trees with a wide distribution, limited information is available for the interaction between the endangeredPseudotsugatrees and EcM fungi, especially the spore bank. The aim of this study was to investigate EcM fungal spore bank communities in soil in remnant patches of Japanese Douglas-fir (Pseudotsuga japonica) forest. We conducted a bioassay of 178 soil samples collected from threeP. japonicaforests and their neighboring arbuscular mycorrhizal artificial plantations, using the more readily available North American Douglas-fir (Pseudotsuga menziesii) as bait seedlings. EcM fungal species were identified by a combination of morphotyping and DNA sequencing of the ITS region. We found that EcM fungal spore banks were present not only inP. japonicaforests but also in neighboring plantations. Among the 13 EcM fungal species detected,Rhizopogon togasawariushad the second highest frequency and was found in all plots, regardless of forest type. Species richness estimators differed significantly among forest types. The community structure of EcM fungal spore banks differed significantly between study sites but not between forest types. These results indicate that EcM fungal spore banks are not restricted to EcM forests and extend to surrounding forest dominated by arbuscular mycorrhizal trees, likely owing to the durability of EcM fungal spores in soils.