Organization of the chick CDC37 gene
Organization of the chick CDC37 gene
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DOI：
10.1074/jbc.273.6.3598
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发表时间：
1998-02-06
影响因子：
4.8
通讯作者：
Toole, BP
中科院分区：
文献类型：
--
作者：
Huang, L;Grammatikakis, N;Toole, BP
CDC 37和伴侣蛋白Hsp 90形成结合几种激酶的复合物，导致其活性的稳定和促进。CDC 37还以序列特异性方式结合DNA和糖胺聚糖。在这项研究中，我们进一步描述了鸡CDC 37和CDC 37基因的组织。鸡CDC 37是一种类似于50-kDa的蛋白质，由一种类似于1.7的内切酶的mRNA编码。CDC 37基因类似于8.5内切酶，包含8个外显子和7个不同大小的内含子。假定的启动子和5 '侧翼区含有E2盒和SP 1、S8同源结构域蛋白和髓系祖细胞转录因子MZF 1内的两个锌指簇的共有结合位点。特别引人注目的是由高度重复的10-11-碱基对序列组成的类似于470-碱基对区域，（T/C）gCTAT（A/G）GGG（A/T）（其中g表示存在于11-碱基对序列中的额外G）。该区域包括15个拷贝的序列TAT-GGGGA，其符合由MZF 1中的锌指簇之一识别的DNA共有序列。这些发现强调了CDC 37在组织发育和重组过程中调节细胞行为的潜在重要性。
CDC37 and the chaperone protein, Hsp90, form a complex that binds to several kinases, resulting in stabilization and promotion of their activity. CDC37 also binds DNA and glycosaminoglycans in a sequence-specific manner. In this study, we further characterize chick CDC37 and examine the organization of the CDC37 gene. Chick CDC37 is a similar to 50-kDa protein encoded by an mRNA of similar to 1.7 kilobases. The CDC37 gene is similar to 8.5 kilobases and contains 8 exons and 7 introns of various sizes. The presumptive promoter and 5'-flanking regions contain an E2 box and consensus binding sites for SP1, for the S8 homeodomain protein, and for two zinc finger clusters within the myeloid progenitor transcription factor, MZF1. Particularly striking is a similar to 470-base pair region composed of a highly repetitive 10-11-base pair sequence, (T/C)gCTAT(A/G)GGG(A/T) (where g represents the additional G present in the 11-base pair sequence). This region includes 15 copies of the sequence, TAT-GGGGA, which conforms to the DNA consensus sequence recognized by one of the zinc finger clusters in MZF1. These findings emphasize the potential importance of CDC37 in regulation of cellular behavior during tissue development and reorganization.