Enantioselective fractionation of fluoroquinolones in the aqueous environment using chiral liquid chromatography coupled with tandem mass spectrometry
Enantioselective fractionation of fluoroquinolones in the aqueous environment using chiral liquid chromatography coupled with tandem mass spectrometry
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DOI：
10.1016/j.chemosphere.2018.05.005
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发表时间：
2018-09-01
期刊：
影响因子：
8.8
通讯作者：
Kasprzyk-Hordern, Barbara
中科院分区：
文献类型：
--
作者：
Castrignano, Erika;Kannan, Andrew M.;Kasprzyk-Hordern, Barbara
采用手性HPLC-MS/MS方法和CHIR-ALCEL (R) OZ-RH色谱柱研究了（氟）喹诺酮类药物及其代谢物在固体和液体环境基质中的多残基对映体谱。对手性氧氟沙星及其主要代谢物氧氟沙星- n-氧化物和去甲基氧氟沙星进行了同时手性分离；莫西沙星;前药普卢利沙星及其活性化合物乌利沙星；氟甲喹;纳地沙星和R-(+)-贝西沙星。非手性抗生素（环丙沙星、诺氟沙星和纳利地酸）也被纳入该方法，以便在一次分析中分析所有靶向喹诺酮类药物。8种手性药物中有5种获得了满意的对映体分辨率（Rs >= 1），可以进行定量分析。该方法的总体性能令人满意，大多数分析物的方法精密度为70%，方法检出限（MDL）为低ng L-1水平(0.1 < MDL (ng L-1)< 6.4, 0.1 < MDL (ng L-1)< 6.6和0.1
This paper aims to examine the multiresidue enantiomeric profiling of (fluoro)quinolones and their metabolites in solid and liquid environmental matrices using chiral HPLC-MS/MS method and a CHIR-ALCEL (R) OZ-RH column. Simultaneous chiral separation was obtained for chiral ofloxacin and its main metabolites ofloxacin-N-oxide and desmethyl-ofioxacin; moxifloxacin; the prodrug prulifloxacin and its active compound ulifloxacin; flumequine; nadifloxacin and R-(+)-besifloxacin. Achiral antibiotics (ciprofloxacin, norfloxacin and nalidixic acid) were also included in the method to enable the analysis of all targeted quinolones within one analytical run. Satisfactory enantiomeric resolution (Rs >= 1) was obtained for five out of eight chiral drugs enabling quantitative analysis. The overall performance of the method was satisfactory with a method precision 70% for most of the analytes and method detection limits (MDL) at low ng L-1 levels (0.1 < MDL (ng L-1)< 6.4, 0.1 < MDL (ng L-1)< 6.6 and 0.1