Discovery of powdery mildew resistance gene candidates from Aegilops biuncialis chromosome 2Mb based on transcriptome sequencing
Discovery of powdery mildew resistance gene candidates from Aegilops biuncialis chromosome 2Mb based on transcriptome sequencing
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基于转录组测序从山羊草染色体2Mb中发现白粉病抗性候选基因
DOI：
10.1371/journal.pone.0220089
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发表时间：
2019-11-11
期刊：
影响因子：
3.7
通讯作者：
Liu, Wenxuan
中科院分区：
文献类型：
--
作者：
Li, Huanhuan;Dong, Zhenjie;Liu, Wenxuan
白粉病是小麦最常见的病害之一。开发和部署抗性品种是防治该病害最经济、最有效的方法之一。我们前期的研究表明，中国春(CS)-山羊草2M(b)二体附加系TA7733中的2M(b)基因赋予小麦高水平的白粉病抗性。在本研究中，使用 15 株 Blumeria graminis f 测定了 TA7733 的耐药谱。 sp。小麦（Bgt）分离株在中国不同地区流行。结果表明，TA7733对所有测试的Bgt分离株均具有高度抗性，并且染色体2M(b)上的基因赋予对白粉病的广谱抗性。为了通过鉴定源自伊蚊的候选 R 基因来表征白粉病抗性机制。 biuncialis 染色体 2M(b) 并开发 2M(b) 特异性分子标记，我们对 TA7733 和 CS 进行了 RNA-seq 分析。我们总共鉴定了 7,278 个 unigenes，与 CS 相比，它们在 Bgt 感染前后的 TA7733 中显示出特异性表达。在这 7,278 个 unigene 中，295 个被注释为假定的抗性 (R) 基因。使用来自 TA7733 和 CS 的 R 基因序列的比较分析和整合 CS Ref Seq v1.0 来开发 R 基因特异性引物。在 295 个 R 基因中，我们鉴定出 53 个 R 基因是 2M(b) 特异的，可能与白粉病抗性有关。对编码核苷酸结合富含亮氨酸重复序列 (NLR) 蛋白的 53 个 R 基因中的大多数进行功能注释。 TA7733 对白粉病的广谱抗性和本研究中确定的 2M(b) 衍生的假定候选 R 基因特异性分子标记的可用性将为通过诱导 CS-Ae 将白粉病抗性从 2M(b) 转移到普通小麦奠定基础。 biuncialis同源重组。我们的研究还为进一步分离和克隆伊蚊的白粉病抗性基因提供了有用的候选者。双颊染色体 2M(b)。
Powdery mildew is one of the most widespread diseases of wheat. The development and deployment of resistant varieties are one of the most economical and effective methods to manage this disease. Our previous study showed that the gene(s) at 2M(b) in Chinese Spring (CS)-Aegilops biuncialis 2M(b) disomic addition line TA7733 conferred a high level of resistance to powdery mildew of wheat. In this study, resistance spectrum of TA7733 was assayed by using 15 Blumeria graminis f. sp. tritici (Bgt) isolates prevalent in different regions of China. The result indicated that TA7733 was highly resistant to all tested Bgt isolates and the gene(s) on chromosome 2M(b) conferred broad-spectrum resistance to powdery mildew. In order to characterize mechanism of powdery mildew resistance by identifying candidates R-genes derived from Ae. biuncialis chromosome 2M(b) and develop 2M(b)-specific molecular markers, we performed RNA-seq analysis on TA7733 and CS. In total we identified 7,278 unigenes that showed specific expression in TA7733 pre and post Bgt-infection when compared to CS. Of these 7,278 unigenes, 295 were annotated as putative resistance (R) genes. Comparatively analysis of R-gene sequences from TA7733 and CS and integration CS Ref Seq v1.0 were used to develop R-gene specific primers. Of 295 R-genes we identified 53 R-genes were specific to 2M(b) and could be involved in powdery mildew resistance. Functional annotation of majority of the 53 R-genes encoded nucleotide binding leucine rich repeat (NLR) protein. The broad-spectrum resistance to powdery mildew in TA7733 and availability of 2M(b)-derived putative candidate R-gene specific molecular markers identified in this study will lay foundations for transferring powdery mildew resistance from 2M(b) to common wheat by inducing CS-Ae. biuncialis homoeologous recombination. Our study also provides useful candidates for further isolation and cloning of powdery mildew resistance gene(s) from Ae. biuncialis chromosome 2M(b).