Drosophila tracheal system formation involves FGF‐dependent cell extensions contacting bridge‐cells
Drosophila tracheal system formation involves FGF‐dependent cell extensions contacting bridge‐cells
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DOI：
10.1093/embo-reports/kvf115
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发表时间：
2002-06
期刊：
影响因子：
7.7
通讯作者：
C. Wolf;Nina Gerlach;R. Schuh
中科院分区：
文献类型：
--
作者：
C. Wolf;Nina Gerlach;R. Schuh
外胚层来源的果蝇气管系统的发展是基于分支的生长和融合，将异构化排列的气管亚单位相互连接成高度定型的三维管状结构。最近的研究表明，这一过程涉及中胚层起源的一种特殊类型的细胞，称为桥细胞。单桥细胞位于相邻的气管亚基之间，是向外生长的背干分支的引导柱。我们发现，接近于桥细胞的气管细胞形成丝状细胞延伸，它附着在桥细胞表面，对于气管亚单位的相互连接是必不可少的。显性-阴性实验和功能获得实验的结果都表明，细胞延伸的形成需要Cdc42介导的果蝇成纤维细胞生长因子活性。
Development of the ectodermally derived Drosophila tracheal system is based on branch outgrowth and fusion that interconnect metamerically arranged tracheal subunits into a highly stereotyped three‐dimensional tubular structure. Recent studies have revealed that this process involves a specialized cell type of mesodermal origin, termed bridge‐cell. Single bridge‐cells are located between adjacent tracheal subunits and serve as guiding posts for the outgrowing dorsal trunk branches. We show that bridge‐cell‐approaching tracheal cells form filopodia‐like cell extensions, which attach to the bridge‐cell surface and are essential for the tracheal subunit interconnection. The results of both dominant‐negative and gain‐of‐function experiments suggest that the formation of cell extensions require Cdc42‐mediated Drosophila fibroblast growth factor activity.