Pokeweed mitogen induced differentiation of human B cells: evaluation by a protein A haemolytic plaque assay.
Pokeweed mitogen induced differentiation of human B cells: evaluation by a protein A haemolytic plaque assay.
复制标题
美洲商陆有丝分裂原诱导人 B 细胞分化：通过蛋白 A 溶血斑测定进行评估。
作者：
L. Hammarström;A. Bird;S. Britton;C. I. Smith
使用蛋白A空斑试验，测定美洲商陆有丝分裂原刺激后分泌各类免疫球蛋白的人细胞的数量。在最佳应答时（第5-7天），当使用外周血淋巴细胞时，发现平均58，354 IgM PFC/10（6）、34，207 IgG PFC/10（6）和10，525伊加PFC/10（6）细胞。在脾细胞中，在第4-6天获得的峰值略高于血液中的峰值。发现分泌任一类型轻链的细胞的比例与未刺激细胞的比例相当，从而支持反应的多克隆性的概念。美洲商陆有丝分裂原刺激外周血淋巴细胞被认为是完全T细胞依赖性的，而脾细胞的反应不是。当在有丝分裂原刺激的培养物中测定抗原特异性前体细胞时，发现SRBC特异性IgM产生细胞的频率为1.3/1000个细胞。这种频率通常被发现是独立的培养基补充。
Using the protein A plaque assay, the number of human cells secreting immunoglobulin of various classes after pokeweed mitogen stimulation was determined. At optimal response (on day 5-7) a mean of 58,354 IgM PFC/10(6), 34,207 IgG PFC/10(6) and 10,525 IgA PFC/10(6) cells was found when using peripheral blood lymphocytes. In spleen cells, peak values which were slightly higher than in blood, were obtained at day 4-6. The proportions of cells secreting light chains of either type were found to be comparable to those of unstimulated cells thus supporting the notion of the polyclonality of the response. Pokeweed mitogen stimulation of peripheral blood lymphocytes was found to be totally T-cell dependent whereas the response of spleen cells was not. When assayed for antigen-specific precursor cells in cultures stimulated by mitogen, the frequency of SRBC-specific IgM producing cells was found to be 1.3/1000 cells. This frequency was regularly found to be independent of medium supplement.