Effect of capillary shear stress on recovery and osteogenic differentiation of muscle-derived precursor cell populations
Effect of capillary shear stress on recovery and osteogenic differentiation of muscle-derived precursor cell populations
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DOI：
10.1002/term.355
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发表时间：
2011-08-01
影响因子：
3.3
通讯作者：
Wall, Ivan
中科院分区：
文献类型：
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作者：
Mulhall, Hayley;Patel, Minal;Wall, Ivan
化学和物理刺激都可以影响前体细胞群体的命运。因此，必须了解它们对促进不需要的分化事件的影响，以提高再生医学方法的治疗细胞的产量和纯度。毛细管剪切力，类似于细胞处理过程中遇到的那些，可以影响再生细胞群的产生。由于剪切力可以促进粘附的骨髓源性基质细胞的成骨分化，我们试图确定是否同样适用于从肌肉生态位环境中分离的肌肉源性前体细胞（MDPC）群体。我们从5天大的皇家外科学院大鼠的颅面肌中分离MDPC，并使它们经受与在细胞的手动生物处理期间遇到的那些类似的毛细血管剪切事件。然后我们评估MDPCs的活力和异位成骨分化是否受到影响。我们发现，虽然立即恢复的MDPC没有显着影响剪切，活力24小时后减少相比，非剪切MDPC。到48小时，剪切的MDPC已全部恢复，并具有与未剪切的MDPC相似的活力。在成骨和成肌培养基中暴露于毛细血管剪切后，成骨分化增强。这表明，与用于治疗的细胞群的生物加工过程中遇到的剪切力类似的剪切力可以对MDPC的命运产生显著影响。版权所有（C）2010约翰威利父子有限公司
Both chemical and physical stimuli can influence the fate of precursor cell populations. Therefore, the impact they have on promoting unwanted differentiation events must be understood to improve the yield and purity of therapeutic cells for regenerativemedicine approaches. Capillary shear forces, similar to those encountered during cell processing, can impact upon production of regenerative cell populations. As shear stress can promote osteogenic differentiation in adhered bonemarrow-derived stromal cells, we sought to determine whether the same is true for populations of muscle-derived precursor cells (MDPCs) that were isolated from a muscle niche environment. We isolated MDPCs from craniofacial muscle of 5 day-old Royal College of Surgeons rats and subjected them to capillary shear events similar to those encountered during manual bioprocessing of cells. We then assessed whether viability and ectopic osteogenic differentiation of MDPCs was affected. We found that whilst immediate recovery of MDPCs was not significantly affected by shear, viability after 24 h was reduced in comparison to non-sheared MDPCs. By 48 h, sheared MDPCs had all recovered and had similar viability to non-sheared MDPCs. Ostegenic differentiation was enhanced following exposure to capillary shear in both osteogenic and myogenic medium. This indicates that shear forces similar to those encountered during the bioprocessing of cell populations for therapy can have a significant influence on the fate of MDPCs. Copyright (C) 2010 John Wiley & Sons, Ltd.