Potential role of lipin-1 in exercise-induced mitochondrial biogenesis
Potential role of lipin-1 in exercise-induced mitochondrial biogenesis
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DOI：
10.1016/j.bbrc.2008.07.079
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发表时间：
2008-09-26
影响因子：
3.1
通讯作者：
Terada, Shin
中科院分区：
文献类型：
--
作者：
Higashida, Kazuhiko;Higuchi, Mitsuru;Terada, Shin
耐力运动诱导骨骼肌线粒体生物合成。研究表明，lipin-1在肝脏中充当转录辅激活因子，刺激线粒体酶的基因表达。我们推测lipin-1可能参与运动诱导的骨骼肌线粒体生物合成。本研究首次证实，大鼠三头肌中的lipin-1 mRNA在急性一轮耐力游泳运动后增加了约2倍。第二，L 6肌管中lipin-1的异位表达增加了肉毒碱棕榈酰转移酶-1和δ-氨基乙酰丙酸合成酶基因的表达。最后，在皮下注射5-氨基咪唑-4-甲酰胺核糖核苷（AICAR）或克伦特罗（分别是5 '-AMP-活化蛋白激酶（AMPK）和β 2-肾上腺素能受体（β 2-AR）活化剂）后6 h，大鼠三头肌中lipin-1 mRNA的表达显著升高。这些结果可能表明，lipin-1的表达增强参与运动诱导的线粒体酶适应，可能通过AMPK和β 2-AR相关机制。(C)2008年爱思唯尔公司All rights reserved.
Endurance exercise induces mitochondrial biogenesis in skeletal muscle. It has been shown that lipin-1 acts as a transcriptional coactivator in liver, and Stimulates gene expression of mitochondrial enzymes. We hypothesized that lipin-1 might be involved in exercise-induced mitochondrial biogenesis in skeletal muscle. The present investigation first demonstrated that lipin-1 mRNA in rat triceps muscle was increased by approximately 2-fold after an acute bout of endurance swimming exercise. Second, ectopic expression of lipin-1 in L6 myotube increased carnitine palmitoyltransferase-1 and delta-aminolevulinate synthase gene expression. Finally, lipin-1 mRNA expression in rat triceps muscle was significantly elevated at 6 h after subcutaneous injections of 5-aminomidazole-4-carboxamide ribonucleciside (AICAR) or clenbuterol, which are 5'-AMP-activated protein kinase (AMPK) and beta 2-adrenergic receptor (beta 2-AR) activators, respectively. These results may suggest that enhanced expression of lipin-1 is involved in exercise-induced mitochondrial enzyme adaptations, possibly through AMPK- and beta 2-AR-related mechanisms. (C) 2008 Elsevier Inc. All rights reserved.