FGF signaling mediates definitive endoderm formation by regulating epithelial-to-mesenchymal transition and cell proliferation
FGF signaling mediates definitive endoderm formation by regulating epithelial-to-mesenchymal transition and cell proliferation
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DOI：
10.1387/ijdb.190372ql
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Li Qiuhong
中科院分区：
文献类型：
--
作者：
Li shengbiao;Huang Qingsong;Mao Jianwen;Li Qiuhong
FGF signaling pathway is imperative for de nitive endoderm (DE) differentiation from human embryonic stem cells (hESCs), which always accompanies an epithelial-to-mesenchymal transition (EMT) process. However, whether there is an association between FGF signaling and the EMT during DE formation in vitro has remained elusive. In the present study, we identify that several FGF family members were signi cantly activated during the differentiation of hESCs toward DE. Inhibition of FGF signaling by an ef cient and selective inhibitor BGJ398 abolishes both the EMT and DE induction by blocking the activation of the zinc- nger transcription factor SNAI1 which is a direct transcriptional repressor of cell adhesion protein CDH1. In addition, cell proliferation is also severely in uenced by attenuating the FGF signaling. Collectively, we propose that the FGF signaling promotes the DE formation through mediating the EMT and cell proliferation.
