Nucleolar antigen found in several human tumors but not in the nontumor tissues studied.
Nucleolar antigen found in several human tumors but not in the nontumor tissues studied.
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在几种人类肿瘤中发现了核仁抗原，但在所研究的非肿瘤组织中没有发现。
DOI：
10.1073/pnas.76.2.892
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发表时间：
1979
影响因子：
11.1
通讯作者：
H. Busch
中科院分区：
文献类型：
--
作者：
F. Davis;F. Gyorkey;R. Busch;H. Busch
用间接免疫荧光技术检测从HeLa细胞中分离的兔抗核仁抗体在HeLa细胞中产生明亮的核仁荧光。经胎牛血清和胎盘细胞核吸收后，在12种人肿瘤细胞，包括HeLa、Hep-2、前列腺癌和乳腺癌培养物、Goldenberg GW-39结肠癌、前列腺癌、肾上腺皮质癌、甲状腺癌和鳞癌、脾毛细胞白血病、结肠腺癌肝转移和骨肉瘤的活检标本中，仍能产生明亮的核仁荧光。9种非肿瘤细胞包括骨髓、肾脏、胎盘、甲状腺、肝脏和前列腺的活检标本、外周血棕黄色涂层和正常皮肤成纤维细胞的培养均未产生明亮的核仁荧光。在HeLa细胞中，酸、碱和蛋白水解酶可阻止核仁荧光，而核酸酶不能阻止；HeLa核仁提取液吸收此抗体可阻止HeLa和其他人类肿瘤细胞的核色荧光。
Rabbit antibodies to nucleoli isolated from HeLa cells produced bright nucleolar fluorescence in HeLa cells by the indirect immunofluorescence technique. After absorption with fetal bovine serum and placental nuclei, the IgG still produced bright nucleolar fluorescence in 12 human tumor cells including HeLa, HEp-2, cultures of prostate and mammary carcinomas, the Goldenberg GW-39 colon tumor, and biopsy specimens of prostatic, adrenal cortical, thyroid, and squamous cell carcinomas, a hairy cell leukemia of the spleen, a hepatic metastasis of an adenocarcinoma of the colon, and an osteogenic sarcoma. Bright nucleolar fluorescence was not produced in nine nontumor human cells including biopsy specimens of bone marrow, kidney, placenta, thyroid, liver, and prostate, peripheral blood buffy coat, and cultures of normal skin fibroblasts. Nucleolar fluorescence with the absorbed IgG was prevented in HeLa cells by pretreatment of the cells with acid, base, and proteases but not by pretreatment with nucleases; absorption of this IgG with extracts of HeLa nucleoli prevented the nucleolor fluorescence in HeLa and other human tumor cells.