Effects of mild calorie restriction on lipid metabolism and inflammation in liver and adipose tissue
Effects of mild calorie restriction on lipid metabolism and inflammation in liver and adipose tissue
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DOI：
10.1016/j.bbrc.2017.06.090
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发表时间：
2017-08-26
影响因子：
3.1
通讯作者：
Han, Sung Nim
中科院分区：
文献类型：
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作者：
Park, Chan Yoon;Park, Soyoung;Han, Sung Nim
卡路里限制(CR)被报道可以改善脂代谢和减少炎症性疾病。然而，大多数现有的CR模型使用30%-50%的卡路里减少，这在人类身上很难实现。我们研究了轻度CR对脂代谢和炎症反应的影响。雄性C57BL/6小鼠随机饲喂对照饲料(10%大卡脂肪，Control)或高脂饲料(60%大卡脂肪，HFD)，或减少对照组饲料，以达到15%CR，持续16周。CR组的体重、白色脂肪组织重量、肝脏三酰甘油水平和血清胎球蛋白-A水平均低于对照组。CR组血清脂联素水平高于对照组，HFD组低于对照组。CR组大鼠肝脏和脂肪组织MCP-1mRNA水平显著低于对照组。HFD组脂肪组织MCP-1、11-6、TNF-α和SOCS3的mRNA水平显著高于对照组和CR组，且与血清脂联素水平呈负相关。CR组瘦素水平最低，肝脏Lepr表达最高，Lepr基因表达水平与肝脏SOCS3基因表达水平呈正相关。我们的研究结果表明，轻度CR可降低肥胖，从而导致更高的脂联素和更低的胎球蛋白-A水平，并可能有助于减轻肝脏和脂肪组织的炎症状态。此外，轻度CR可能通过上调Lepr的表达而影响瘦素的敏感性。(C)2017 Elsevier Inc.保留所有权利。
Calorie restriction (CR) has been reported to improve lipid metabolism and to decrease inflammatory diseases. However, most existing CR models use 30-50% calorie reduction, which is hard to achieve in humans. We investigated the effects of mild CR on lipid metabolism and inflammatory responses. Male C57BL/6 mice were fed control diet (10% kcal fat, Control) or high fat diet (60% kcal fat, HFD) ad libitum or reduced amount of control diet to achieve 15% CR for 16 wks. Body weights, white adipose tissue weights, liver triacylglycerol levels, and serum fetuin-A levels were lower in CR than in the Control. Serum adiponectin levels were higher in CR and lower in HFD compared with the Control. Liver and adipose tissue Mcp-1 mRNA levels were significantly lower in CR compared with the Control. Adipose tissue mRNA levels of Mcp-1, 11-6, Tnf-alpha and Socs3 were significantly higher in HFD than in the Control and CR, and levels of these negatively correlated with serum adiponectin levels. CR group had the lowest leptin levels and the highest liver Lepr expression, and Lepr mRNA levels positively correlated with liver Socs3 mRNA levels. Our findings showed that mild CR lowered adiposity which resulted in higher adiponectin and lower fetuin-A levels, and might have contributed to alleviation of inflammatory status in the liver and adipose tissue. Furthermore, mild CR might have affected leptin sensitivity by up-regulating Lepr expression. (C) 2017 Elsevier Inc. All rights reserved.