GLYCOSYL PHOSPHATIDYL-INOSITOL PHOSPHOLIPASE-D ACTIVITY IN HUMAN SERUM
GLYCOSYL PHOSPHATIDYL-INOSITOL PHOSPHOLIPASE-D ACTIVITY IN HUMAN SERUM
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DOI：
10.1177/000456329503200107
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发表时间：
1995-01-01
影响因子：
2.2
通讯作者：
GOSSNER, A
中科院分区：
文献类型：
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作者：
MAGUIRE, GA;GOSSNER, A
用血清碱性磷酸酶释放活性测定健康人和疾病人血清糖基磷脂酰肌醇磷脂酶D(GPI-PLD)。血清浓度的线性关系仅在血清容量非常低的情况下才能获得(L，约0.2亩)，这需要对血清进行大量预稀释，以避免潜在的伪影。该分析的精密度足以满足常规使用的要求。肝病患者的活性低于健康对照组，而肾脏疾病患者的活性高于健康对照组。肝移植后，GPI-PLD与常规肝功能指标无相关性，但与胆固醇浓度有显着相关性。这些观察结果表明，肝脏是血清中GPI-PLD的主要来源。它的功能仍不清楚。
We measured serum glycosyl phosphatidyl inositol phospholipase D (GPI-PLD) by its alkaline phosphatase releasing activity in healthy and diseased individuals. Linearity with respect to serum concentration was obtained only with very low serum volumes (below about 0.2 mu L) necessitating a large predilution of serum to avoid potential artefacts. The assay was sufficiently precise for routine use. Patients with liver disease had lower activities and those with renal disease had higher activities than healthy controls. Following liver transplantation there was no correlation between GPI-PLD and conventional markers of liver function but there was a marked correlation with cholesterol concentration. These observations suggest that liver is a major source of GPI-PLD in serum. Its function remains unknown.