Regeneration of transformed plants from hairy roots of Plumbago indica
Regeneration of transformed plants from hairy roots of Plumbago indica
复制标题
DOI：
10.1007/s11240-010-9702-z
复制
发表时间：
2010-07-01
影响因子：
3
通讯作者：
Bhattacharya, Sabita
中科院分区：
文献类型：
--
作者：
Gangopadhyay, Moumita;Chakraborty, Dipjyoti;Bhattacharya, Sabita
发根农杆菌诱导的白花菜毛状根在MS液体培养基中连续光照培养3周后，观察到不定芽的自发再生。经聚合酶链式反应和测序证实，该基因已插入到转化植株中。转化植株在含L(-1)6-苄基腺嘌呤0.5 mg的MS固体培养基上培养1周后，移栽到无生长调节剂的培养基上，植株的总体生长情况好于对照植株。与未转化植株相比，转化植株具有更高的根系生物量和更高的白花素含量。
Spontaneous shoot regeneration was observed from Agrobacterium rhizogenes-induced hairy roots of Plumbago indica when these were incubated in liquid MS medium for a period of 3 weeks under continuous light. Insertion of the rolB gene in putative transformed plants was confirmed by PCR and sequencing. Transformed plants grown for a period of 1 week on solid MS medium containing 0.5 mg l(-1) 6-benzyladenine and then transplanted to growth regulator-free medium showed better overall growth than control plants. Transformed plants had a higher root bio-biomass and an increased plumbagin content relative to non-transformed plants.