The expression of Gal beta 1,4GlcNAc alpha 2,6 sialyltransferase and alpha 2,6-linked sialoglycoconjugates in human brain tumors
The expression of Gal beta 1,4GlcNAc alpha 2,6 sialyltransferase and alpha 2,6-linked sialoglycoconjugates in human brain tumors
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DOI：
10.1007/s004010050427
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发表时间：
1996-03-01
影响因子：
12.7
通讯作者：
Moskal, JR
中科院分区：
文献类型：
--
作者：
Kaneko, Y;Yamamoto, H;Moskal, JR
CMP-NeuAc：Galβ1，4GlcNAcα2，6-唾液酸基转移酶(α2，6-ST)[EC 2.4.99.1]受发育调控，具有高度的组织特异性，在肿瘤的转化和转移中起重要作用。在本研究中，我们首次详细分析了α-2，6-ST和α-2，6-唾液酸偶联物在人脑肿瘤中的表达。我们用抗大鼠α2，6-ST的多克隆单特异性抗体和α2，6-唾液酸特异性凝集素--接骨木凝集素(SNA)进行组织化学研究，并用人α2，6-ST特异的cDNADNA探针进行Northern分析。脑膜瘤、脊索瘤和颅咽管瘤经常表达α2，6-ST和α2，6-联结唾液酸糖结合物。在不同的脑膜瘤亚型中，脑膜上皮型脑膜瘤的抗α2，6-ST抗体和SNA染色均较纤维母细胞型和间变性脑膜瘤强。另一方面，所有胶质起源的肿瘤和髓母细胞瘤实际上都不表达α2，6-ST或α2，6-联结唾液糖结合物。此外，在脑转移瘤中观察到α2，6-ST和α2，6-联结唾液酸糖结合物的表达非常微弱或可以忽略不计。综上所述，α2，6-ST和α2，6-联结唾液糖共轭表达与非神经外胚层上皮样肿瘤有关。
CMP-NeuAc: Gal beta 1,4GlcNAc alpha 2,6 sialyltransferase (alpha 2,6-ST) [EC 2.4.99.1] is developmentally regulated, shows a high degree of tissue specificity, and appears to play a role in oncogenic transformation and metastasis. In the present study, we have performed the first detailed analysis of the expression of alpha 2,6-ST and alpha 2,6-linked sialoglycoconjugates in human brain tumors. We used a polyclonal, monospecific anti-rat alpha 2,6-ST antibody and the alpha 2,6-linked sialic acid-specific lectin, Sambucus nigra agglutinin (SNA) for histochemical studies, and a human alpha 2,6-ST-specific cDNA probe for Northern analysis. Meningiomas, chordomas and craniopharyngiomas frequently expressed alpha 2,6-ST and alpha 2,6-linked sialoglycoconjugates. Among the different meningioma subtypes, meningothelial meningiomas stained more strongly with both anti-alpha 2,6-ST antibody and SNA than the fibroblastic and anaplastic meningiomas. On the other hand, all tumors of glial origin and medulloblastomas were virtually devoid of either alpha 2,6-ST or alpha 2,6-linked sialoglycoconjugate expression. Moreover, very weak to negligible expression of both alpha 2,6-ST and alpha 2,6-linked sialoglycoconjugates was observed in brain metastases. In conclusion, alpha 2,6-ST and alpha 2,6-linked sialoglycoconjugate expression is associated with non-neuroectodermal epithelial-like tumors.