Conditioned medium alters electrophysiological and transmitter-related properties expressed by rat enteric neurons in cell culture.
Conditioned medium alters electrophysiological and transmitter-related properties expressed by rat enteric neurons in cell culture.
复制标题
条件培养基改变细胞培养物中大鼠肠神经元表达的电生理学和递质相关特性。
DOI：
10.1016/0306-4522(88)90035-8
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发表时间：
1988
期刊：
影响因子：
3.3
通讯作者：
Willard,AL
中科院分区：
文献类型：
--
作者：
Nishi,R;Willard,AL
我们以前已经表明，大鼠肠神经元显示出许多他们在体内表型时，他们被解离和长期细胞培养生长。为了评估这些表型的可塑性的程度，我们已经检查了大鼠心脏细胞的条件培养基的效果，因为这种治疗强烈影响大鼠交感神经元在培养中的递质特性。肠神经元在条件培养基中生长3-4周引起了几种变化，这些变化类似于先前描述的条件培养基对培养物中其他神经元细胞类型的影响。当与在对照培养基中生长的培养物相比时，在条件培养基中生长的培养物：（i）含有3倍的大（> 25 μm）神经元;（ii）合成和储存3-4倍的乙酰胆碱;（iii）含有4-5倍的具有可检测的5-羟色胺免疫反应性的神经元;（iv）在持续去极化过程中，神经元的数目增加了10倍，并且还观察到了一些在其他系统中未见报道的变化。条件培养基培养物：（i）含有较少的神经元突起和可化学检测到的血管活性肠多肽、P物质、生长抑素和[Met]脑啡肽;（ii）含有70%的具有血管活性肠多肽样免疫反应性的神经元胞体;和（iii）含有四倍于乙酰胆碱的毒蕈碱反应神经元。上述性质的变化并不均匀地影响所有肠神经元，即使在条件培养基中生长6周后也是如此。我们的结论是，肠神经元表型的异质性是建立在出生前，限制了某些子集的神经元在环境中的外源性因素作出反应的能力。然而，当暴露于条件培养基时，其他神经元亚群的表型显示出相当大的可塑性。
We have previously shown that rat enteric neurons display many of theirin vivophenotypes when they are dissociated and grown in long-term cell culture. To assess the degree of plasticity of these phenotypes we have examined the effect of medium conditioned by rat heart cells because this treatment strongly affects transmitter properties in rat sympathetic neurons in culture. Growth of enteric neurons for 3–4 weeks in conditioned medium caused several changes that are similar to previously described effects of conditioned medium on other neuronal cell types in culture. When compared to cultures grown in control medium, cultures grown in conditioned medium: (i) contained three times as many large (> 25 μm) neurons; (ii) synthesized and stored 3–4 times as much acetylcholine; (iii) contained 4–5 times as many neurons with detectable 5-hydroxytryptamine immunoreactivity; and (iv) contained 10 times as many neurons that fired repetitively during sustained depolarization.Several other changes, which have not been reported in other systems, were also observed. Conditioned medium cultures: (i) contained many fewer neuronal processes with immunohistochemically detectable vasoactive intestinal polypeptide, substance P, somatostatin, and [Met]enkephalin; (ii) contained 70% fewer neuronal cell bodies with vasoactive intestinal polypeptide-like immunoreactivity; and (iii) contained four times as many neurons that had muscarinic responses to acetylcholine.None of the changes in properties described above uniformly affected all enteric neurons, even after 6 weeks of growth in conditioned medium. We conclude that the heterogeneity of enteric neuron phenotypes is established prior to birth and limits the capacity of certain subsets of neurons to respond to exogenous factors in the environment. Nevertheless, the phenotypes of other subsets of neurons displayed considerable plasticity when exposed to conditioned medium.