A novel Cry1A resistance allele of fall armyworm in the new invaded region.
A novel Cry1A resistance allele of fall armyworm in the new invaded region.
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DOI：
10.1016/j.ijbiomac.2023.125392
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发表时间：
2023-06
影响因子：
8.2
通讯作者：
M. Jin;Yinxue Shan;Qi Li;Yan Peng;Yutao Xiao
中科院分区：
文献类型：
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作者：
M. Jin;Yinxue Shan;Qi Li;Yan Peng;Yutao Xiao
草地贪夜蛾（Spodoptera frugiperda）是其原产地西半球的一种毁灭性害虫，并已成为地球仪的主要入侵害虫。转Bt毒素基因作物已被广泛用于防治。食虫类。然而，抗性的进化威胁着Bt作物的可持续性。场演化在美洲发现了对Bt作物的田间抗性，而在新入侵的东半球没有田间抗性的报道。本文研究了一株抗Cry 1Ab的猪链球菌LZ-R株的分子机制。frugiperda在我国玉米田采集后，利用Cry 1Ab进行了27代的选择。LZ-R株与SfABCC 2-KO株的互补试验表明，LZ-R株与SfABCC 2-KO株的F1代对Cry 1Ab的抗性水平相近，说明LZ-R株中存在SfABCC 2的共同突变位点。通过对LZ-R株SfABCC 2基因cDNA全序列的测定，发现了一个新的突变等位基因。交叉抗性结果表明，Cry 1Ab抗性菌株对Cry 1F也具有>260倍的抗性，而对Vip 3A没有交叉抗性。这些结果提供了在新入侵的南半球中存在一种新的SfABCC 2突变等位基因的证据。食虫类。
The fall armyworm,Spodoptera frugiperda, is a devastating pest in its native range Western Hemisphere and has become a major invasive pest around the globe. Transgenic crops producing Bt toxins have been widely used to controlS. frugiperda. However, the evolution of resistance threatens the sustainability of Bt crops. Field-evolvedS. frugiperdaresistance to Bt crops was observed in America, whereas, no case of field-resistance was reported in its newly invaded East Hemisphere. Here we investigated the molecular mechanism of a Cry1Ab-resistant LZ-R strain ofS. frugiperda, which selected 27-generations using Cry1Ab after being collected in corn fields from China. Complementation tests between LZ-R strain and SfABCC2-KO strain, which have been knockoutSfABCC2gene and confer 174-fold resistance to Cry1Ab, showed a similar level of resistance in the F1-progeny as their parent stains, indicating that a common locus ofSfABCC2mutation in LZ-R stain. Sequencing of the full length ofSfABCC2cDNA from LZ-R strain, we characterize a novel mutation allele ofSfABCC2. Cross-resistance results showed that Cry1Ab-resistance strain also confers >260-fold resistance to Cry1F, with no cross-resistance to Vip3A. These results provided evidence of a novelSfABCC2mutation allele in the newly invaded East Hemisphere ofS. frugiperda.