c-Jun expression in adult rat dorsal root ganglion neurons: Differential response after central or peripheral axotomy
c-Jun expression in adult rat dorsal root ganglion neurons: Differential response after central or peripheral axotomy
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DOI：
10.1006/exnr.1997.6665
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发表时间：
1997-11-01
影响因子：
5.3
通讯作者：
Bregman, BS
中科院分区：
文献类型：
--
作者：
Broude, E;McAtee, M;Bregman, BS
成熟的中枢神经系统（CNS）对损伤的反应与周围神经系统（PNS）的反应有显著不同。轴突切断的PNS神经元通常在损伤后再生，而CNS神经元不再生。造成这些差异的机制尚不完全清楚，但内在的神经元和外在的环境影响可能有助于再生的成功或失败。一个可能有助于成功轴突再生的内在因素是在受损神经元中诱导特定基因。在本研究中，我们已经评估的假设，即立即早期基因c-jun的表达参与了一个成功的再生反应。我们比较了c-Jun在背根神经节（DRG）神经元中枢或外周轴突切断后的表达。我们准备了接受坐骨神经（外周）损伤或背根切断术联合脊髓半切（中枢损伤）的动物。在第三组动物中，将切断的背根与胎儿脊髓移植物一起沿着置于半切部位。这种干预已被证明可以促进切断的轴突的再生，并提供了一个模型，以检查DRG神经元在中枢损伤后的再生生长。我们的研究结果表明，c-Jun在DRG神经元的外周轴突切断后大幅上调，但在中央轴突切断后，只有18%的神经元表达c-Jun。背根切断和移植后，c-Jun的表达显着上调;在这些实验条件下，63%的DRG神经元c-Jun阳性。这些数据表明，c-Jun表达可能与PNS和CNS损伤后的成功再生生长有关。(C)北京：科学出版社.
The response of the mature central nervous system (CNS) to injury differs significantly from the response of the peripheral nervous system (PNS). Axotomized PNS neurons generally regenerate following injury, while CNS neurons do not. The mechanisms that are responsible for these differences are not completely known, but both intrinsic neuronal and extrinsic environmental influences are likely to contribute to regenerative success or failure. One intrinsic factor that may contribute to successful axonal regeneration is the induction of specific genes in the injured neurons. In the present study, we have evaluated the hypothesis that expression of the immediate early gene c-jun is involved in a successful regenerative response. We have compared c-Jun expression in dorsal root ganglion (DRG) neurons following central or peripheral axotomy. We prepared animals that received either a sciatic nerve (peripheral) lesion or a dorsal rhizotomy in combination with spinal cord hemisection (central lesion). In a third group of animals, severed dorsal roots were placed into the hemisection site along with a fetal spinal cord transplant. This intervention has been demonstrated to promote regrowth of severed axons and provides a model to examine DRG neurons during regenerative growth after central lesion. Our results indicated that c-Jun was upregulated substantially in DRG neurons following a peripheral axotomy, but following a central axotomy, only 18% of the neurons expressed c-Jun. Following dorsal rhizotomy and transplantation, however, c-Jun expression was upregulated dramatically; under those experimental conditions, 63% of the DRG neurons were c-Jun-positive. These data indicate that c-Jun expression may be related to successful regenerative growth following both PNS and CNS lesions. (C) 1997 Academic Press.