Influence of manganese on cell morphology, protoplasts formation and β-D-glucosidase activity in Phaffia rhodozyma
Influence of manganese on cell morphology, protoplasts formation and β-D-glucosidase activity in Phaffia rhodozyma
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DOI：
10.1016/s0378-1097(98)00595-3
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发表时间：
1999-02-15
影响因子：
2.1
通讯作者：
Callieri, DA
中科院分区：
文献类型：
--
作者：
Pera, LM;Rubinstein, L;Callieri, DA
在几种潜在的细胞壁裂解酶中，如β-D-葡萄糖苷酶、β-D-半乳糖苷酶、β-N-乙酰-D-氨基葡萄糖苷酶、β-N-乙酰-D-氨基半乳糖苷酶和β-D-N，N '-二乙酰几丁质酶，红球藻仅显示β-D-葡萄糖苷酶活性。MnCl 2对酶活性有促进作用，但对酶产量无影响。当酵母与4.0 mM MnCl 2一起生长时，细胞比不存在MnCl 2时获得的细胞更大更圆，表明细胞壁弱化。使用0.3 M KCl作为渗透稳定剂，从这些细胞中获得原生质体。(C)1999年，欧洲微生物学会联合会。由Elsevier Science B. V.出版，版权所有。
Among several potential cell wall lytic enzymes, such as beta-D-glucosidase, beta-D-galactosidase, beta-N-acetyl-D-glucosaminidase, beta-N-acetyl-D-galactosaminidase, and beta-D-N,N'-diacetylchitinase, Phaffia rhodozyma showed only beta-D-glucosidase activity. MnCl2 increased the activity, but not the production of the enzyme. When the yeast was grown with 4.0 mM MnCl2, the cells were bigger and rounder than those obtained in its absence, suggesting a weakening of the cell wall. Protoplasts were obtained from these cells, using 0.3 M KCl as the osmotic stabiliser. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.