Biochemical markers of type II collagen breakdown and synthesis are positioned at specific sites in human osteoarthritic knee cartilage
Biochemical markers of type II collagen breakdown and synthesis are positioned at specific sites in human osteoarthritic knee cartilage
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DOI：
10.1016/j.joca.2007.09.006
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发表时间：
2008-05-01
影响因子：
7
通讯作者：
Delaisse, J. -M.
中科院分区：
文献类型：
--
作者：
Bay-Jensen, A. -C.;Andersen, T. L.;Delaisse, J. -M.
目的：探讨是否可以在 CA 软骨组织的特定组织学特征水平上检测体液中用于骨关节炎（OA）活动评估的 II 型胶原转换标记物，以及它们在该水平上的相互关系。方法：从 32 个 CA 膝关节的全深度软骨活检中获取相邻切片。对 Helix-II 和 CTX-II 进行了免疫组织化学分析，Helix-II 和 CTX-II 分别是源自三螺旋和端肽区域的 II 型胶原蛋白片段，被认为反映了不同的分解事件，以及 IIA N 型前肽 (PIIANP)，这是一种反映 IIA 型胶原蛋白合成的生化标记物。 结果：Helix-II 和 CTX-II 在先前报道的胶原蛋白损伤区域中检测到，最常见于软骨细胞周围，但也经常在非胶原蛋白损伤区域中检测到。以前研究过诸如边缘区域和靠近软骨下骨的区域，包括血管化部位和骨-软骨界面。后者是CTX-II 的主导地位，很少出现Helix-If。 PIIANP 与 Helix-II 和 CTX-II 在有限数量的特征上共定位，主要是在深部软骨中。总体而言，我们的分析强调了标记物与特定组织学特征的清晰关联模式，并表明它们以有序的方式扩散到这些特征。结论：Helix-II 和 CTX-II 对软骨组织中的特定特征表现出一定程度的差异选择性。靠近骨的 CTX-II 检测可能与软骨下骨在 OA 中的可能作用有关。分解标记物和 PIIANP 的有限共定位表明胶原蛋白片段只能部分由新合成的胶原蛋白产生。我们的研究增强了人们对以下问题的兴趣：将反映不同区域软骨贡献或代谢过程的几种标记物组合起来是否应该能够更广泛地检测 OA 活动。 (C) 2007 年国际骨关节炎研究协会。由爱思唯尔有限公司出版。保留所有权利。
Objective: To investigate whether type II collagen turnover markers used for osteoarthritis (OA) activity evaluation in body fluids can be detected at the level of specific histological features of CA cartilage tissue, as well as how they relate with each other at this level.Methods: Adjacent sections were obtained from full-depth cartilage biopsies from 32 CA knees. Immunohistochemistry was performed for Helix-II and CTX-II, which are type II collagen fragments originating from the triple helix and the telopeptide region, respectively, and believed to reflect distinct breakdown events, as well as for type IIA N propeptide (PIIANP), a biochemical marker reflecting synthesis of type IIA collagen.Results: Helix-II and CTX-II were detected in areas where collagen damage was reported previously, most frequently around chondrocytes, but also frequently in regions not previously investigated such as the margin area and close to subchondral bone, including vascularization sites and bone-cartilage interface. The latter is CTX-II's prevailing position and shows rarely Helix-If. PIIANP co-localized with Helix-II and CTX-II on a limited number of features, mainly in deep zone cartilage. Overall, our analysis highlights clear patterns of association of the markers with specific histological features, and shows that they spread to these features in an ordered way.Conclusion: Helix-II and CTX-II show to some degree differential selectivity for specific features in cartilage tissue. CTX-II detection close to bone may be relevant to the possible role of subchondral bone in OA. The restricted co-localization of breakdown markers and PIIANP suggests that collagen fragments can result only partially from newly synthesized collagen. Our study strengthens the interest for the question whether combining several markers reflecting different regional cartilage contributions or metabolic processes should allow a broader detection of OA activity. (C) 2007 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.